MELICHEROVÁ, Janka, Jana ILGOVÁ, Martin KVÁČ, Břetislav KOUDELA and Andrea VALIGUROVÁ. LIFE CYCLE OF CRYPTOSPORIDIUM MURIS IN TWO RODENTS EXHIBITING A DIFFERENT RESPONSE TO THE PARASITIZATION. In Iveta Hodová a Iva Přikrylová. X. české a slovenské parazitologické dny. Brno: Masarykova univerzita, Brno 2012, 2012. p. 74-206. ISBN 978-80-210-5862-0.
Other formats:   BibTeX LaTeX RIS
Basic information
Authors MELICHEROVÁ, Janka (703 Slovakia, belonging to the institution), Jana ILGOVÁ (703 Slovakia, belonging to the institution), Martin KVÁČ (203 Czech Republic), Břetislav KOUDELA (203 Czech Republic, belonging to the institution) and Andrea VALIGUROVÁ (703 Slovakia, guarantor, belonging to the institution).
Edition Brno, X. české a slovenské parazitologické dny, p. 74-206, 2012.
Publisher Masarykova univerzita, Brno 2012
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
RIV identification code RIV/00216224:14310/12:00058221
Organization unit Faculty of Science
ISBN 978-80-210-5862-0
Keywords in English cryptosporidia; development; gastric; merozoite; oocyst; pathology
Tags AKR, rivok
Tags Reviewed
Changed by Changed by: Mgr. Janka Melicherová, učo 381738. Changed: 17. 2. 2014 23:20.
Genus Cryptosporidium belongs to the phylum Apicomplexa comprising numerous important human and animal pathogens. Considering their unique epicellular location on the surface of host epithelial cells as well as molecular analyses pointing out their phylogenetic affinity with gregarines, cryptosporidia are recently often excluded from the typical coccidia. Cryptosporidium muris is a gastrointestinal pathogen parasitizing epithelial cells within crypts of the gastric glands and exhibiting a monoxenous life cycle. Main aim of this study was to map the in vivo development of C. muris using a combined microscopic approach and molecular tool (nested PCR amplifying SSU rRNA.Experimental inoculations with infective oocysts were performed simultaneously in two species of laboratory rodents, BALB/c mice and the southern multimammate rat Mastomys coucha, differing in their prepatent and patent period. Emphasis was given to the existence and the chronology of particular developmental stages that are generally described throughout the literature related to cryptosporidia. The presence of merozoites Type II and the thin walled oocysts, that are thought to be responsible for autoinfection, was confirmed in both rodent models; nevertheless, they occurred in these hosts at different time after inoculation and in different abundance. The initial phase of the infection of BALB/c mice progressed rapidly with a prepatent period of 7-10 days, while in M. coucha the developmental stages of C. muris were observed later and its prepatent period was longer (18-21 days). Similarly, the patent periods of BALB/c mice and M. coucha differed significantly, i.e. 10-15 days and chronic infection throughout the life of host respectively. We focused on pathological alterations of the affected gastric tissue. In M. coucha, the first pathological modifications were observed in a chronic phase of infection, while the gastric tissue of BALB/c mice exhibited no observable changes induced by the parasite.
GPP506/10/P372, research and development projectName: Srovnávací morfologie a imunohistochemie v hodnocení fylogenetických vztahů mezi zástupci raných linií kmene Apicomplexa
Investor: Czech Science Foundation
PrintDisplayed: 20. 5. 2022 23:10