BRÁZDA, Václav, Jana ČECHOVÁ, Jan COUFAL, Sigrun RUMPEL and Eva BRÁZDOVÁ JAGELSKÁ. Superhelical DNA as a preferential binding target of 14-3-3 gamma protein. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS. PHILADELPHIA: TAYLOR & FRANCIS INC, 2012, vol. 30, No 4, p. 371-378. ISSN 0739-1102. Available from: https://dx.doi.org/10.1080/07391102.2012.682205. |
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@article{1082677, author = {Brázda, Václav and Čechová, Jana and Coufal, Jan and Rumpel, Sigrun and Brázdová Jagelská, Eva}, article_location = {PHILADELPHIA}, article_number = {4}, doi = {http://dx.doi.org/10.1080/07391102.2012.682205}, keywords = {14-3-3 proteins; DNA binding; protein-DNA complex; superhelical DNA}, language = {eng}, issn = {0739-1102}, journal = {JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS}, title = {Superhelical DNA as a preferential binding target of 14-3-3 gamma protein}, volume = {30}, year = {2012} }
TY - JOUR ID - 1082677 AU - Brázda, Václav - Čechová, Jana - Coufal, Jan - Rumpel, Sigrun - Brázdová Jagelská, Eva PY - 2012 TI - Superhelical DNA as a preferential binding target of 14-3-3 gamma protein JF - JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS VL - 30 IS - 4 SP - 371-378 EP - 371-378 PB - TAYLOR & FRANCIS INC SN - 07391102 KW - 14-3-3 proteins KW - DNA binding KW - protein-DNA complex KW - superhelical DNA N2 - The 14-3-3 protein family is a highly conserved and widely distributed group of proteins consisting of multiple isoforms in eukaryotes. Ubiquitously expressed, 14-3-3 proteins play key roles in DNA replication, cell cycle regulation, and apoptosis. The function of 14-3-3 proteins is mediated by interaction with a large number of other proteins and with DNA. It has been demonstrated that 14-3-3 gamma protein binds strongly to cruciform structures and is crucial for initiating replication. In this study, we analyzed DNA binding properties of the 14-3-3 gamma isoform to linear and supercoiled DNA. We demonstrate that 14-3-3 gamma protein binds strongly to long DNA targets, as evidenced by electrophoretic mobility shift assay on agarose gels. Binding of 14-3-3 gamma to DNA target results in the appearance of blurry, retarded DNA bands. Competition experiments with linear and supercoiled DNA on magnetic beads show very strong preference for supercoiled DNA. We also show by confocal microscopy that 14-3-3 protein in the HCT-116 cell line is co-localized with DNA cruciforms. This implies a role for the 14-3-3 gamma protein in its binding to local DNA structures which are stabilized by DNA supercoiling. ER -
BRÁZDA, Václav, Jana ČECHOVÁ, Jan COUFAL, Sigrun RUMPEL and Eva BRÁZDOVÁ JAGELSKÁ. Superhelical DNA as a preferential binding target of 14-3-3 gamma protein. \textit{JOURNAL OF BIOMOLECULAR STRUCTURE \&{}amp; DYNAMICS}. PHILADELPHIA: TAYLOR \&{}amp; FRANCIS INC, 2012, vol.~30, No~4, p.~371-378. ISSN~0739-1102. Available from: https://dx.doi.org/10.1080/07391102.2012.682205.
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