Conjugation of 5(6)-carboxyfluorescein and
5(6)-carboxynaphthfluorescein with bovine serum albumin and
their immobilization for optical pH sensing

J 2012

Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

BIDMANOVÁ, Šárka, Antonín HLAVÁČEK, Jiří DAMBORSKÝ and Zbyněk PROKOP

Basic information

Original name

Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

Authors

BIDMANOVÁ, Šárka (203 Czech Republic, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Zbyněk PROKOP (203 Czech Republic, belonging to the institution)

Edition

SENSORS AND ACTUATORS B: CHEMICAL, 2012, 0925-4005

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.535

RIV identification code

RIV/00216224:14310/12:00062930

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.snb.2011.09.046

UT WoS

000301549400007

Keywords in English

pH indicator Fluorescence Optical sensor Immobilization Conjugation Fluorescein

Abstract

V originále

An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol–gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0–9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring. Immobilized conjugates of 5(6)-carboxynaphthofluorescein and bovine serum albumin showed an enhanced sensitivity in alkaline pH levels, response for glutaraldehyde crosslinking ranged from pH 9.1 to 11.0 and from 8.3 to 10.0 for the ORMOCER entrapment, however, control of the indicator leaching was essential.

Links

ED0001/01/01, research and development project

Name: CETOCOEN

Citovat

BIDMANOVÁ, Šárka, Antonín HLAVÁČEK, Jiří DAMBORSKÝ and Zbyněk PROKOP. Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing. SENSORS AND ACTUATORS B: CHEMICAL. 2012, vol. 161, No 1, p. 93-99. ISSN 0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2011.09.046.

@article{1082690,
   author = {Bidmanová, Šárka and Hlaváček, Antonín and Damborský, Jiří and Prokop, Zbyněk},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.snb.2011.09.046},
   keywords = {pH indicator Fluorescence Optical sensor Immobilization Conjugation Fluorescein},
   language = {eng},
   issn = {0925-4005},
   journal = {SENSORS AND ACTUATORS B: CHEMICAL},
   title = {Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing},
   volume = {161},
   year = {2012}
}

TY  - JOUR
ID  - 1082690
AU  - Bidmanová, Šárka - Hlaváček, Antonín - Damborský, Jiří - Prokop, Zbyněk
PY  - 2012
TI  - Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing
JF  - SENSORS AND ACTUATORS B: CHEMICAL
VL  - 161
IS  - 1
SP  - 93-99
EP  - 93-99
SN  - 09254005
KW  - pH indicator Fluorescence Optical sensor Immobilization Conjugation Fluorescein
N2  - An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol–gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0–9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring. Immobilized conjugates of 5(6)-carboxynaphthofluorescein and bovine serum albumin showed an enhanced sensitivity in alkaline pH levels, response for glutaraldehyde crosslinking ranged from pH 9.1 to 11.0 and from 8.3 to 10.0 for the ORMOCER entrapment, however, control of the indicator leaching was essential.
ER  -

BIDMANOVÁ, Šárka, Antonín HLAVÁČEK, Jiří DAMBORSKÝ and Zbyněk PROKOP. Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing. \textit{SENSORS AND ACTUATORS B: CHEMICAL}. 2012, vol.~161, No~1, p.~93-99. ISSN~0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2011.09.046.

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