J 2012

Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

BIDMANOVÁ, Šárka, Antonín HLAVÁČEK, Jiří DAMBORSKÝ and Zbyněk PROKOP

Basic information

Original name

Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing

Authors

BIDMANOVÁ, Šárka (203 Czech Republic, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Zbyněk PROKOP (203 Czech Republic, belonging to the institution)

Edition

SENSORS AND ACTUATORS B: CHEMICAL, 2012, 0925-4005

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.535

RIV identification code

RIV/00216224:14310/12:00062930

Organization unit

Faculty of Science

UT WoS

000301549400007

Keywords in English

pH indicator Fluorescence Optical sensor Immobilization Conjugation Fluorescein

Tags

Tags

International impact, Reviewed
Změněno: 22/4/2013 15:19, Ing. Andrea Mikešková

Abstract

V originále

An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol–gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0–9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring. Immobilized conjugates of 5(6)-carboxynaphthofluorescein and bovine serum albumin showed an enhanced sensitivity in alkaline pH levels, response for glutaraldehyde crosslinking ranged from pH 9.1 to 11.0 and from 8.3 to 10.0 for the ORMOCER entrapment, however, control of the indicator leaching was essential.

Links

ED0001/01/01, research and development project
Name: CETOCOEN