BIDMANOVÁ, Šárka, Antonín HLAVÁČEK, Jiří DAMBORSKÝ and Zbyněk PROKOP. Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing. SENSORS AND ACTUATORS B: CHEMICAL. 2012, vol. 161, No 1, p. 93-99. ISSN 0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2011.09.046.
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Basic information
Original name Conjugation of 5(6)-carboxyfluorescein and 5(6)-carboxynaphthfluorescein with bovine serum albumin and their immobilization for optical pH sensing
Authors BIDMANOVÁ, Šárka (203 Czech Republic, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Zbyněk PROKOP (203 Czech Republic, belonging to the institution).
Edition SENSORS AND ACTUATORS B: CHEMICAL, 2012, 0925-4005.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.535
RIV identification code RIV/00216224:14310/12:00062930
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1016/j.snb.2011.09.046
UT WoS 000301549400007
Keywords in English pH indicator Fluorescence Optical sensor Immobilization Conjugation Fluorescein
Tags AKR, rivok
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 22/4/2013 15:19.
Abstract
An approach for the immobilization of a pH indicator in optical pH sensors and biosensors was developed. Fluorescent dyes 5(6)-carboxyfluorescein and 5(6)-carboxynaphthofluorescein were conjugated with bovine serum albumin as a non-enzymatic scaffold protein and the conjugation procedure was optimized. Fluorescent properties, sensitivity to temperature and photostability of conjugates have been studied and characterized into details. The conjugates were immobilized on glass support by: (i) glutaraldehyde crosslinking or (ii) entrapment in sol–gel matrix ORMOCER with subsequent glutaraldehyde cross-linking. The response to pH and leaching potential were evaluated. The sensor layer, based on immobilized conjugates of 5(6)-carboxyfluorescein and bovine serum albumin, displayed rapid response over the pH range 4.0–9.0, making it compatible with a range of applications such as bioprocessing, clinical diagnostics or environmental monitoring. Immobilized conjugates of 5(6)-carboxynaphthofluorescein and bovine serum albumin showed an enhanced sensitivity in alkaline pH levels, response for glutaraldehyde crosslinking ranged from pH 9.1 to 11.0 and from 8.3 to 10.0 for the ORMOCER entrapment, however, control of the indicator leaching was essential.
Links
ED0001/01/01, research and development projectName: CETOCOEN
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