NEDĚLA, Vilém, Eva TIHLAŘÍKOVÁ, Aleš HAMPL and Miroslava SEDLÁČKOVÁ. SEM and ESEM Observation of Stem Cells. Comparative Study of Human Embryonic Stem Cell Morphology. Imaging & Microscopy. Darmstadt: GIT Verlag, 2012, vol. 2012, No 4, p. 32-34. ISSN 1439-4243.
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Basic information
Original name SEM and ESEM Observation of Stem Cells. Comparative Study of Human Embryonic Stem Cell Morphology.
Authors NEDĚLA, Vilém (203 Czech Republic, guarantor), Eva TIHLAŘÍKOVÁ (203 Czech Republic), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Miroslava SEDLÁČKOVÁ (203 Czech Republic, belonging to the institution).
Edition Imaging & Microscopy, Darmstadt, GIT Verlag, 2012, 1439-4243.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14110/12:00063279
Organization unit Faculty of Medicine
Keywords in English Biological Samples; SEM; ESEM; Human Embryonic Stem Cells
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 14/2/2013 09:08.
Abstract
Studying biological samples with scanning electron microscopy has specific requirements for their preparation. Sample drying is a particularly critical operation for objects such as cultured cells. The requirement for damaging drying step can be eliminated using environmental scanning electron microscopy. This study compares dried and wet samples of cultured human embryonic stem cells. It points to the advantages of both methods and to the complementarity of the information that they provide.
Links
MSM0021622430, plan (intention)Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie
Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
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