HANINEC, Pavel, Radek KAISER, Vladimír BOBEK and Petr DUBOVÝ. Enhancement of musculocutaneous nerve reinnervation after vascular endothelial growth factor (VEGF) gene therapy. BMC Neuroscience. London: Biomed Central Ltd., 2012, vol. 13, No 57, p. "nestránkováno", 11 pp. ISSN 1471-2202. doi:10.1186/1471-2202-13-57.
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Basic information
Original name Enhancement of musculocutaneous nerve reinnervation after vascular endothelial growth factor (VEGF) gene therapy
Authors HANINEC, Pavel (203 Czech Republic), Radek KAISER (203 Czech Republic), Vladimír BOBEK (203 Czech Republic) and Petr DUBOVÝ (203 Czech Republic, guarantor, belonging to the institution).
Edition BMC Neuroscience, London, Biomed Central Ltd. 2012, 1471-2202.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30000 3. Medical and Health Sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.000
RIV identification code RIV/00216224:14740/12:00063476
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1186/1471-2202-13-57
UT WoS 000308692500001
Keywords in English NEURORRHAPHY; STIMULATES AXONAL OUTGROWTH; CILIARY NEUROTROPHIC FACTOR; PERIPHERAL-NERVE; ULNAR NERVE; CELL-SURVIVAL; SCHWANN-CELLS; MOTOR; REGENERATION; REPAIR
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 7. 4. 2013 17:19.
Abstract
Background: Vascular endothelial growth factor (VEGF) is not only a potent angiogenic factor but it also promotes axonal outgrowth and proliferation of Schwann cells. The aim of the present study was to quantitatively assess reinnervation of musculocutaneous nerve (MCN) stumps using motor and primary sensory neurons after plasmid phVEGF transfection and end-to-end (ETE) or end-to-side (ETS) neurorrhaphy. The distal stump of rat transected MCN, was transfected with plasmid phVEGF, plasmid alone or treated with vehiculum and reinnervated following ETE or ETS neurorrhaphy for 2 months. The number of motor and dorsal root ganglia neurons reinnervating the MCN stump was estimated following their retrograde labeling with Fluoro-Ruby and Fluoro-Emerald. Reinnervation of the MCN stumps was assessed based on density, diameter and myelin sheath thickness of regenerated axons, grooming test and the wet weight index of the biceps brachii muscles. Results: Immunohistochemical detection under the same conditions revealed increased VEGF in the Schwann cells of the MCN stumps transfected with the plasmid phVEGF, as opposed to control stumps transfected with only the plasmid or treated with vehiculum. The MCN stumps transfected with the plasmid phVEGF were reinnervated by moderately higher numbers of motor and sensory neurons after ETE neurorrhaphy compared with control stumps. However, morphometric quality of myelinated axons, grooming test and the wet weight index were significantly better in the MCN plasmid phVEGF transfected stumps. The ETS neurorrhaphy of the MCN plasmid phVEGF transfected stumps in comparison with control stumps resulted in significant elevation of motor and sensory neurons that reinnervated the MCN. Especially noteworthy was the increased numbers of neurons that sent out collateral sprouts into the MCN stumps. Similarly to ETE neurorrhaphy, phVEGF transfection resulted in significantly higher morphometric quality of myelinated axons, behavioral test and the wet weight index of the biceps brachii muscles. Conclusion: Our results showed that plasmid phVEGF transfection of MCN stumps could induce an increase in VEGF protein in Schwann cells, which resulted in higher quality axon reinnervation after both ETE and ETS neurorrhaphy. This was also associated with a better wet weight biceps brachii muscle index and functional tests than in control rats.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
NS10496, research and development projectName: Vymezení úspěšnosti end-to-side anastomózy periferních nervů ve vztahu k ostatním neurotizačním technikám v klinické praxi a využití neurotrofických látek aplikovaných ve formě plasmidů v experimentálním modelu
Investor: Ministry of Health of the CR
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