Off-line coupling of automated pipetting system with square
wave voltammetry as a tool for study of drug-DNA interaction

J 2010

Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction

HUSKA, Dalibor; Vojtech ADAM; Jaromír HUBÁLEK; Libuše TRNKOVÁ; Tomáš ECKSCHLAGER et. al.

Basic information

Original name

Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction

Authors

HUSKA, Dalibor (203 Czech Republic); Vojtech ADAM (203 Czech Republic); Jaromír HUBÁLEK (203 Czech Republic); Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution); Tomáš ECKSCHLAGER (203 Czech Republic); Marie STIBOROVÁ (203 Czech Republic); Ivo PROVAZNIK (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)

Edition

Chimica Oggi/Chemistry today, 2010, 1973-8250

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10403 Physical chemistry

Country of publisher

Italy

Confidentiality degree

is not subject to a state or trade secret

RIV identification code

RIV/00216224:14310/10:00063547

Organization unit

Faculty of Science

UT WoS

000283582200005

Keywords in English

ellipticine; DNA; square wave voltammetry; adsorptive transfer technique; automation

Abstract

In the original language

Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interaction between DNA and ellipticine by using of off-line coupled automated pipetting system with adsorptive transfer stripping technique (AdTS) square wave voltammetry (SWV). Interaction between dsDNA and ellipticine was evident from the decrease of CA signal. Six ng of ellipticine per ml resulted in a statistically significant decrease in CA signal (the decrease was higher than the standard deviation of determinations, Student t-test). The decrease in CA signal corresponded to increasing ellipticine concentrations.

Links

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

Cite

HUSKA, Dalibor; Vojtech ADAM; Jaromír HUBÁLEK; Libuše TRNKOVÁ; Tomáš ECKSCHLAGER; Marie STIBOROVÁ; Ivo PROVAZNIK and René KIZEK. Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction. Chimica Oggi/Chemistry today. 2010, vol. 28, No 5, p. 18-20. ISSN 1973-8250.

@article{1085566,
   author = {Huska, Dalibor and Adam, Vojtech and Hubálek, Jaromír and Trnková, Libuše and Eckschlager, Tomáš and Stiborová, Marie and Provaznik, Ivo and Kizek, René},
   article_number = {5},
   keywords = {ellipticine; DNA; square wave voltammetry; adsorptive transfer technique; automation},
   language = {eng},
   issn = {1973-8250},
   journal = {Chimica Oggi/Chemistry today},
   title = {Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction},
   volume = {28},
   year = {2010}
}

TY  - JOUR
ID  - 1085566
AU  - Huska, Dalibor - Adam, Vojtech - Hubálek, Jaromír - Trnková, Libuše - Eckschlager, Tomáš - Stiborová, Marie - Provaznik, Ivo - Kizek, René
PY  - 2010
TI  - Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction
JF  - Chimica Oggi/Chemistry today
VL  - 28
IS  - 5
SP  - 18-20
EP  - 18-20
SN  - 19738250
KW  - ellipticine
KW  - DNA
KW  - square wave voltammetry
KW  - adsorptive transfer technique
KW  - automation
N2  - Manual handling with DNA and drugs can be a source of errors, which can be eliminated also by automation of whole process of sample preparation. The aim of this work was to study interaction between DNA and ellipticine by using of off-line coupled automated pipetting system with adsorptive transfer stripping technique (AdTS) square wave voltammetry (SWV). Interaction between dsDNA and ellipticine was evident from the decrease of CA signal. Six ng of ellipticine per ml resulted in a statistically significant decrease in CA signal (the decrease was higher than the standard deviation of determinations, Student t-test). The decrease in CA signal corresponded to increasing ellipticine concentrations.
ER  -

HUSKA, Dalibor; Vojtech ADAM; Jaromír HUBÁLEK; Libuše TRNKOVÁ; Tomáš ECKSCHLAGER; Marie STIBOROVÁ; Ivo PROVAZNIK and René KIZEK. Off-line coupling of automated pipetting system with square wave voltammetry as a tool for study of drug-DNA interaction. \textit{Chimica Oggi/Chemistry today}. 2010, vol.~28, No~5, p.~18-20. ISSN~1973-8250.

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