The PI3K/AKT Signaling Pathway Controls the Quiescence of the
Low-Rhodamine123-Retention Cell Compartment Enriched for
Melanoma Stem Cell Activity

J 2013

The PI3K/AKT Signaling Pathway Controls the Quiescence of the Low-Rhodamine123-Retention Cell Compartment Enriched for Melanoma Stem Cell Activity

TOUIL, Yasmine, Thomas ZULIANI, Isabelle WOLOWCZUK, Klaudia KURANDA, Jiřina MEDALOVÁ et. al.

Basic information

Original name

The PI3K/AKT Signaling Pathway Controls the Quiescence of the Low-Rhodamine123-Retention Cell Compartment Enriched for Melanoma Stem Cell Activity

Authors

TOUIL, Yasmine (250 France), Thomas ZULIANI (250 France), Isabelle WOLOWCZUK (250 France), Klaudia KURANDA (250 France), Jiřina MEDALOVÁ (203 Czech Republic, guarantor, belonging to the institution), Joris ANDRIEUX (250 France), Helene LE ROY (250 France), Laurent MORTIER (250 France), Jerome VANDOMME (250 France), Nathalie JOUY (250 France), Bernadette MASSELOT (250 France), Pascaline SEGARD (250 France), Bruno QUESNEL (250 France), Pierre FORMSTECHER (250 France) and Renata POLAKOWSKA (250 France)

Edition

Stem Cells, 2013, 1066-5099

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.133

RIV identification code

RIV/00216224:14310/13:00067735

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/stem.1333

UT WoS

000316624300004

Keywords in English

Melanoma stem cells; quiescence;Rhodamine123 exclusion ; LY294002; PI3K/AKT pathway ; phenotype switching

Abstract

V originále

Melanoma is one of the most aggressive and extremely resistant to conventional therapies neoplasms. Recently, cellular resistance was linked to the cancer stem cell phenotype, still controversial and not well defined. In this study, we utilized a Rhodamine 123 (Rh123) exclusion assay to functionally identify stem-like cells in metastatic human melanomas and melanoma cell lines. We demonstrate that a small subset of Rh123-low-retention (Rh123low) cells is enriched for stem cell-like activities, including the ability to self-renew and produce non-stem Rh123high progeny and to form melanospheres, recapitulating the phenotypic profile of the parental tumor.

Citovat

TOUIL, Yasmine, Thomas ZULIANI, Isabelle WOLOWCZUK, Klaudia KURANDA, Jiřina MEDALOVÁ, Joris ANDRIEUX, Helene LE ROY, Laurent MORTIER, Jerome VANDOMME, Nathalie JOUY, Bernadette MASSELOT, Pascaline SEGARD, Bruno QUESNEL, Pierre FORMSTECHER and Renata POLAKOWSKA. The PI3K/AKT Signaling Pathway Controls the Quiescence of the Low-Rhodamine123-Retention Cell Compartment Enriched for Melanoma Stem Cell Activity. Stem Cells. 2013, vol. 31, No 4, p. 641-651. ISSN 1066-5099. Available from: https://dx.doi.org/10.1002/stem.1333.

@article{1085862,
   author = {Touil, Yasmine and Zuliani, Thomas and Wolowczuk, Isabelle and Kuranda, Klaudia and Medalová, Jiřina and Andrieux, Joris and Le Roy, Helene and Mortier, Laurent and Vandomme, Jerome and Jouy, Nathalie and Masselot, Bernadette and Segard, Pascaline and Quesnel, Bruno and Formstecher, Pierre and Polakowska, Renata},
   article_number = {4},
   doi = {http://dx.doi.org/10.1002/stem.1333},
   keywords = {Melanoma stem cells; quiescence;Rhodamine123 exclusion ; LY294002; PI3K/AKT pathway ; phenotype switching},
   language = {eng},
   issn = {1066-5099},
   journal = {Stem Cells},
   title = {The PI3K/AKT Signaling Pathway Controls the Quiescence of the Low-Rhodamine123-Retention Cell Compartment Enriched for Melanoma Stem Cell Activity},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/stem.1333/abstract;jsessionid=47911ED53003824F3EA04EBCBD5A08C7.d02t04},
   volume = {31},
   year = {2013}
}

TY  - JOUR
ID  - 1085862
AU  - Touil, Yasmine - Zuliani, Thomas - Wolowczuk, Isabelle - Kuranda, Klaudia - Medalová, Jiřina - Andrieux, Joris - Le Roy, Helene - Mortier, Laurent - Vandomme, Jerome - Jouy, Nathalie - Masselot, Bernadette - Segard, Pascaline - Quesnel, Bruno - Formstecher, Pierre - Polakowska, Renata
PY  - 2013
TI  - The PI3K/AKT Signaling Pathway Controls the Quiescence of the Low-Rhodamine123-Retention Cell Compartment Enriched for Melanoma Stem Cell Activity
JF  - Stem Cells
VL  - 31
IS  - 4
SP  - 641-651
EP  - 641-651
SN  - 10665099
KW  - Melanoma stem cells
KW  - quiescence;Rhodamine123 exclusion
KW  - LY294002
KW  - PI3K/AKT pathway
KW  - phenotype switching
UR  - http://onlinelibrary.wiley.com/doi/10.1002/stem.1333/abstract;jsessionid=47911ED53003824F3EA04EBCBD5A08C7.d02t04
L2  - http://onlinelibrary.wiley.com/doi/10.1002/stem.1333/abstract;jsessionid=47911ED53003824F3EA04EBCBD5A08C7.d02t04
N2  - Melanoma is one of the most aggressive and extremely resistant to conventional therapies neoplasms. Recently, cellular resistance was linked to the cancer stem cell phenotype, still controversial and not well defined. In this study, we utilized a Rhodamine 123 (Rh123) exclusion assay to functionally identify stem-like cells in metastatic human melanomas and melanoma cell lines. We demonstrate that a small subset of Rh123-low-retention (Rh123low) cells is enriched for stem cell-like activities, including the ability to self-renew and produce non-stem Rh123high progeny and to form melanospheres, recapitulating the phenotypic profile of the parental tumor.
ER  -

TOUIL, Yasmine, Thomas ZULIANI, Isabelle WOLOWCZUK, Klaudia KURANDA, Jiřina MEDALOVÁ, Joris ANDRIEUX, Helene LE ROY, Laurent MORTIER, Jerome VANDOMME, Nathalie JOUY, Bernadette MASSELOT, Pascaline SEGARD, Bruno QUESNEL, Pierre FORMSTECHER and Renata POLAKOWSKA. The PI3K/AKT Signaling Pathway Controls the Quiescence of the Low-Rhodamine123-Retention Cell Compartment Enriched for Melanoma Stem Cell Activity. \textit{Stem Cells}. 2013, vol.~31, No~4, p.~641-651. ISSN~1066-5099. Available from: https://dx.doi.org/10.1002/stem.1333.

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