Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells

BALVAN, Jan, Monika HOLUBOVÁ, Jaromír GUMULEC, Markéta SZTALMACHOVÁ, Martina RAUDENSKÁ, Vojtěch ADAM, René KIZEK and Michal MASAŘÍK. Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells. In MendelNet 2012. 2012.

Basic information

• Original name: Effect of zinc(II) ions on the expression of pro and anti-apoptotic factors in high-grade prostate carcinoma cells
• Authors: BALVAN, Jan, Monika HOLUBOVÁ, Jaromír GUMULEC, Markéta SZTALMACHOVÁ, Martina RAUDENSKÁ, Vojtěch ADAM, René KIZEK and Michal MASAŘÍK.
• Edition: MendelNet 2012, 2012.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: 30200 3.2 Clinical medicine
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Keywords in English: apoptosis;prostate cancer;prognostic marker;Bcl-2;p53;polymerase chain reaction,thiol group;fluorescence microscopy

Abstract

Several typical characteristics of prostate tissue have been identified including the ability to accumulate zinc(II). However, this feature of prostate cells is lost during carcinogenesis and, thus, prostate cells are unable to accumulate zinc(II) ions in high levels. Therefore, we can expect that zinc(II) ions can significantly contribute to the progression of tumour disease and to the ability of prostate cell lines to metastasize. In this study, we aimed our attention on determining the expression of Bcl-2, c-Fos, c-Jun, Ki-67, NF-kB and p53 genes in two prostate cell lines, as the 22Rv1 cell line, a model of aggressive partially androgen-sensitive prostate cancer and the PNT1A cell line, a normal prostate cell line model. Moreover, we were interested in the mechanisms through which exposure of these cell lines to zinc(II) ions could influence expression of the above-mentioned genes. We found that zinc(II) ions caused elevated expression of Ki-67, a marker of proliferation, extremely low expression of p53, high expression of Bcl-2 and no changes in the expression of p53. Our experimental data show different effect of zinc(II) ions on expression of the above-mentioned regulatory genes, which may give us more information on their impact on cancer development and progression with possible using for cancer therapy.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GAP301/10/0356, research and development project: Name: Studie participace specifických mechanismů poškození DNA na cytoxicitě cytostatik vůči lidským chemosensitivním a chemorestentním neuroblastomům