| PLÁNKA, Ladislav, Robert SRNEC, Petr RAUŠER, David STARÝ, Eva FILOVÁ, Josef JANCAR, Jana JUHASOVÁ, Leoš KŘEN, Alois NEČAS and Petr GÁL. Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs. Biomedical Papers. Olomouc: Palacky University, 2012, vol. 156, No 2, p. 128-134. ISSN 1213-8118. doi:10.5507/bp.2012.041. |
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@article{1088030,
author = {Plánka, Ladislav and Srnec, Robert and Raušer, Petr and Starý, David and Filová, Eva and Jancar, Josef and Juhasová, Jana and Křen, Leoš and Nečas, Alois and Gál, Petr},
article_location = {Olomouc},
article_number = {2},
doi = {http://dx.doi.org/10.5507/bp.2012.041},
keywords = {mesenchymal stem cells; growth plate defect; cartilage; bone bridge},
language = {eng},
issn = {1213-8118},
journal = {Biomedical Papers},
title = {Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs},
volume = {156},
year = {2012}
}
TY - JOUR
ID - 1088030
AU - Plánka, Ladislav - Srnec, Robert - Raušer, Petr - Starý, David - Filová, Eva - Jancar, Josef - Juhasová, Jana - Křen, Leoš - Nečas, Alois - Gál, Petr
PY - 2012
TI - Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs
JF - Biomedical Papers
VL - 156
IS - 2
SP - 128-134
EP - 128-134
PB - Palacky University
SN - 12138118
KW - mesenchymal stem cells
KW - growth plate defect
KW - cartilage
KW - bone bridge
N2 - Introduction. This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. Material and methods. The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. Results. Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases. Conclusions. The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.
ER -
PLÁNKA, Ladislav, Robert SRNEC, Petr RAUŠER, David STARÝ, Eva FILOVÁ, Josef JANCAR, Jana JUHASOVÁ, Leoš KŘEN, Alois NEČAS and Petr GÁL. Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs. \textit{Biomedical Papers}. Olomouc: Palacky University, 2012, vol.~156, No~2, p.~128-134. ISSN~1213-8118. doi:10.5507/bp.2012.041.
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