PLÁNKA, Ladislav, Robert SRNEC, Petr RAUŠER, David STARÝ, Eva FILOVÁ, Josef JANCAR, Jana JUHASOVÁ, Leoš KŘEN, Alois NEČAS and Petr GÁL. Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs. Biomedical Papers. Olomouc: Palacky University, 2012, vol. 156, No 2, p. 128-134. ISSN 1213-8118. doi:10.5507/bp.2012.041.
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Basic information
Original name Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs
Authors PLÁNKA, Ladislav, Robert SRNEC, Petr RAUŠER, David STARÝ, Eva FILOVÁ, Josef JANCAR, Jana JUHASOVÁ, Leoš KŘEN, Alois NEČAS and Petr GÁL.
Edition Biomedical Papers, Olomouc, Palacky University, 2012, 1213-8118.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30200 3.2 Clinical medicine
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.990
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.5507/bp.2012.041
UT WoS 000306401700007
Keywords in English mesenchymal stem cells; growth plate defect; cartilage; bone bridge
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 23. 4. 2014 14:43.
Abstract
Introduction. This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. Material and methods. The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. Results. Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases. Conclusions. The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.
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