ŘEMÍNEK, Roman, Jochen PAUWELS, Xu WANG, Jos HOOGMARTENS, Zdeněk GLATZ and Ann VAN SCHEPDAEL. Application of capillary electrophoresis for high-throughput screening of drug metabolism. In Carlos D. García, Karin Y. Chumbimuni-Torres, Emanuel Carrilho. Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations. 1st ed. Hoboken, New Jersey: John Wiley & Sons, Inc. p. 309-317. ISBN 978-0-470-57217-7. doi:10.1002/9781118530009.ch17. 2013.
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Basic information
Original name Application of capillary electrophoresis for high-throughput screening of drug metabolism
Name in Czech Využití kapilární elektroforézy pro testování metabolismu léčiv
Authors ŘEMÍNEK, Roman (203 Czech Republic, belonging to the institution), Jochen PAUWELS (56 Belgium), Xu WANG (156 China), Jos HOOGMARTENS (56 Belgium), Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution) and Ann VAN SCHEPDAEL (56 Belgium).
Edition 1. vyd. Hoboken, New Jersey, Capillary Electrophoresis and Microchip Capillary Electrophoresis: Principles, Applications, and Limitations, p. 309-317, 9 pp. 2013.
Publisher John Wiley & Sons, Inc.
Other information
Original language English
Type of outcome Chapter(s) of a specialized book
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Publication form printed version "print"
WWW URL
RIV identification code RIV/00216224:14310/13:00066018
Organization unit Faculty of Science
ISBN 978-0-470-57217-7
Doi http://dx.doi.org/10.1002/9781118530009.ch17
Keywords (in Czech) kapilární elektroforéza; lidské jaterní mikrosomy; cytochromy P450; vývoj léčiv; stabilita léčiv;
Keywords in English Capillary electrophoresis; human liver microsomes; cytochrome P450 enzyme; drug development; metabolic stability;
Tags AKR, rivok, ZR
Tags International impact, Reviewed
Changed by Changed by: Ing. Andrea Mikešková, učo 137293. Changed: 29/4/2014 16:33.
Abstract
Pharmaceutical industry currently needs to screen extensive compound libraries within pre-clinical stages of drug development. Capillary electrophoresis has become a well-established analytical technique during the last two decades. In spite of the fact that capillary electrophoresis offers a number of favorable features for high-throughput analytical assays, it is still less important than chromatographic techniques in drug development programs. Hence, the main aim of this chapter is to discuss the practical limitations of capillary electrophoresis utilization and suggest ways to overcome these constraints. This is illustrated with a practical example namely the introduction of a method allowing rapid characterization of a compound’s stability against human liver microsomes. NADP+ production monitoring was adopted to ensure generic applicability of the method. The fast stability screening of 12 chosen probe drugs and determination of apparent kinetic parameters for the lidocaine/human liver microsomes system were performed in order to demonstrate the potential of the final method. The results were in a good agreement with the literature data determined by different methods.
Abstract (in Czech)
Byla zavedena metoda pro rychlé stanovení stability léčiv vůči mikrosomálním enzymům. Možnosti metody byly demonstrovány na screeningu 12 vybraných substrátů.
Links
EE2.3.20.0182, research and development projectName: Vytvoření multidisciplinárního výzkumného a vzdělávacího centra bioanalytických technologií
GAP206/10/0057, research and development projectName: Miniaturizovaný systém pro "on-line" studie metabolismu léčiv založený na kapilární elektroforéze
Investor: Czech Science Foundation, Miniaturized on-line drug metabolism system based on capillary electrophoresis
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