| DEMO, Gabriel, Barbora FLIEDROVA, Lenka WEIGNEROVA and Michaela WIMMEROVÁ. Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger. Acta crystallographica. Section F. Hoboken: WILEY-BLACKWELL, 2013, vol. 69, Mar, p. 288-291. ISSN 1744-3091. Available from: https://dx.doi.org/10.1107/S1744309113002522. |
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@article{1089667,
author = {Demo, Gabriel and Fliedrova, Barbora and Weignerova, Lenka and Wimmerová, Michaela},
article_location = {Hoboken},
article_number = {Mar},
doi = {http://dx.doi.org/10.1107/S1744309113002522},
keywords = {Mannosidase; Aspergillus niger; Expression; Crystallization},
language = {eng},
issn = {1744-3091},
journal = {Acta crystallographica. Section F},
title = {Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger},
volume = {69},
year = {2013}
}
TY - JOUR
ID - 1089667
AU - Demo, Gabriel - Fliedrova, Barbora - Weignerova, Lenka - Wimmerová, Michaela
PY - 2013
TI - Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger
JF - Acta crystallographica. Section F
VL - 69
IS - Mar
SP - 288-291
EP - 288-291
PB - WILEY-BLACKWELL
SN - 17443091
KW - Mannosidase
KW - Aspergillus niger
KW - Expression
KW - Crystallization
N2 - beta-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various oligomeric saccharide structures. beta-Mannosidase from Asperigillus niger was expressed in Pichia pastoris and purified to clear homogeneity. beta-mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 A resolution. The crystal belonged to space group P1. Monomeric structure was observed in the asymmetric unit.
ER -
DEMO, Gabriel, Barbora FLIEDROVA, Lenka WEIGNEROVA and Michaela WIMMEROVÁ. Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger. \textit{Acta crystallographica. Section F}. Hoboken: WILEY-BLACKWELL, 2013, vol.~69, Mar, p.~288-291. ISSN~1744-3091. Available from: https://dx.doi.org/10.1107/S1744309113002522.
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