Detailed Information on Publication Record
2013
Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger
DEMO, Gabriel, Barbora FLIEDROVA, Lenka WEIGNEROVA and Michaela WIMMEROVÁBasic information
Original name
Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger
Authors
DEMO, Gabriel (703 Slovakia, belonging to the institution), Barbora FLIEDROVA (203 Czech Republic), Lenka WEIGNEROVA (203 Czech Republic) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Acta crystallographica. Section F, Hoboken, WILEY-BLACKWELL, 2013, 1744-3091
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.568
RIV identification code
RIV/00216224:14740/13:00066027
Organization unit
Central European Institute of Technology
UT WoS
000316745000015
Keywords in English
Mannosidase; Aspergillus niger; Expression; Crystallization
Tags
Tags
International impact, Reviewed
Změněno: 6/4/2014 05:45, Olga Křížová
Abstract
V originále
beta-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various oligomeric saccharide structures. beta-Mannosidase from Asperigillus niger was expressed in Pichia pastoris and purified to clear homogeneity. beta-mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 A resolution. The crystal belonged to space group P1. Monomeric structure was observed in the asymmetric unit.
Links
| ED1.1.00/02.0068, research and development project |
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| GAP207/10/0321, research and development project |
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