Experimental treatment of deep subchondral articular surface
defect of the femur

a 2012

Experimental treatment of deep subchondral articular surface defect of the femur

PLÁNKA, Ladislav, Robert SRNEC, David STARÝ, Lucy VOJTOVÁ, Petr GÁL et. al.

Basic information

Original name

Experimental treatment of deep subchondral articular surface defect of the femur

Name in Czech

Experimentální léčba subchondrálního defektu kloubního povrchu stehenní kosti

Authors

PLÁNKA, Ladislav, Robert SRNEC, David STARÝ, Lucy VOJTOVÁ, Petr GÁL, Jiří KLÍMA and Alois NEČAS

Edition

Journal of Children´s Orthopaedics : Abstracts for 31st EPOS Annual Meeting Helsinki, 18-21 April, 2012, 2012

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30211 Orthopaedics

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Medicine

ISSN

Keywords in English

Femur; Joint, Cartilage, MSCs

Abstract

V originále

Articular cartilage is a tissue with very low regenration ability, therefore the new treatment methods of injuries in this location are constantly sought. Improved healing shows the leasions with current violation of the subchondral bone. Therefore, many treatment methods purposefully violate the subchondral bone, and allowing travel progenitor cells from bone marrow into the defect and ensure at least partial healing (microfracture, forrage). Method This study describes the results of experimental treatment of deep subchondral articular surface defect of the femur (the OCD leason is simulated) with chitosan nanofibers scaffold transplantation staffed mesenchymal stem cells and chondrocytes mixture. Experimental animals were twenty young miniature pigs, the frequency of monitoring 20 weeks. After this period knee arthroscopy evaluation was performed and after the protocol culling of the animal followed the histolgical examination of the healed lesions (O'Driscoll score, Collagen II type, Imunofluorescence - CMDiI). The control group was the same defect created by the stress area of the articular surface of the distal epiphysis of the femur on the other leg, left without treatment. Results Average O'Driscoll score of experimental group A was 15,7 +/- 2,8, in comparison with control group B - O'Driscoll score in average was 4,8 +/- 3,1. In group A higher positivity of Collagen II type (85%) than in group B (25%) was observed as well as in Imunofluorescence (A 80%, B 0%)

Citovat

PLÁNKA, Ladislav, Robert SRNEC, David STARÝ, Lucy VOJTOVÁ, Petr GÁL, Jiří KLÍMA and Alois NEČAS. Experimental treatment of deep subchondral articular surface defect of the femur. In Journal of Children´s Orthopaedics : Abstracts for 31st EPOS Annual Meeting Helsinki, 18-21 April, 2012. 2012. ISSN 1863-2521.

@proceedings{1091903,
   author = {Plánka, Ladislav and Srnec, Robert and Starý, David and Vojtová, Lucy and Gál, Petr and Klíma, Jiří and Nečas, Alois},
   booktitle = {Journal of Children´s Orthopaedics : Abstracts for 31st EPOS Annual Meeting Helsinki, 18-21 April, 2012},
   keywords = {Femur; Joint, Cartilage, MSCs},
   language = {eng},
   title = {Experimental treatment of deep subchondral articular surface defect of the femur},
   url = {http://link.springer.com/content/pdf/10.1007%2Fs11832-012-0390-6.pdf},
   year = {2012}
}

TY  - CONF
ID  - 1091903
AU  - Plánka, Ladislav - Srnec, Robert - Starý, David - Vojtová, Lucy - Gál, Petr - Klíma, Jiří - Nečas, Alois
PY  - 2012
TI  - Experimental treatment of deep subchondral articular surface defect of the femur
KW  - Femur
KW  - Joint, Cartilage, MSCs
UR  - http://link.springer.com/content/pdf/10.1007%2Fs11832-012-0390-6.pdf
N2  - Articular cartilage is a tissue with very low regenration ability, therefore the new treatment methods of injuries in this location are constantly sought. Improved healing shows the leasions with current violation of the subchondral bone. Therefore, many treatment methods purposefully violate the subchondral bone, and allowing travel progenitor cells from bone marrow into the defect and ensure at least partial healing (microfracture, forrage). Method This study describes the results of experimental treatment of deep subchondral articular surface defect of the femur (the OCD leason is simulated) with chitosan nanofibers scaffold transplantation staffed mesenchymal stem cells and chondrocytes mixture. Experimental animals were twenty young miniature pigs, the frequency of monitoring 20 weeks. After this period knee arthroscopy evaluation was performed and after the protocol culling of the animal followed the histolgical examination of the healed lesions (O'Driscoll score, Collagen II type, Imunofluorescence - CMDiI). The control group was the same defect created by the stress area of the articular surface of the distal epiphysis of the femur on the other leg, left without treatment. Results Average O'Driscoll score of experimental group A was 15,7 +/- 2,8, in comparison with control group B - O'Driscoll score in average was 4,8 +/- 3,1. In group A higher positivity of Collagen II type (85%) than in group B (25%) was observed as well as in Imunofluorescence (A 80%, B 0%)
ER  -

PLÁNKA, Ladislav, Robert SRNEC, David STARÝ, Lucy VOJTOVÁ, Petr GÁL, Jiří KLÍMA and Alois NEČAS. Experimental treatment of deep subchondral articular surface defect of the femur. In \textit{Journal of Children´s Orthopaedics : Abstracts for 31st EPOS Annual Meeting Helsinki, 18-21 April, 2012}. 2012. ISSN~1863-2521.

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