VYCHODILOVA, Leona, Jan MATIASOVIC, Olga BOBROVA, Jan FUTAS, Marie KLUMPLEROVA, Karla STEJSKALOVA, Michaela CVANOVÁ, Eva JANOVA, Jarmila OSICKOVA, Mirko VYSKOCIL, Marketa SEDLINSKA, Ladislav DUŠEK, Eliane MARTI a Petr HORIN. Immunogenomic analysis of insect bite hypersensitivity in a model horse population. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY. Amsterdam: ELSEVIER SCIENCE BV, 2013, roč. 152, 3-4, s. 260–268. ISSN 0165-2427. doi:10.1016/j.vetimm.2012.12.013.
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Základní údaje
Originální název Immunogenomic analysis of insect bite hypersensitivity in a model horse population
Autoři VYCHODILOVA, Leona (203 Česká republika, garant), Jan MATIASOVIC (203 Česká republika), Olga BOBROVA (203 Česká republika), Jan FUTAS (203 Česká republika), Marie KLUMPLEROVA (203 Česká republika), Karla STEJSKALOVA (203 Česká republika), Michaela CVANOVÁ (203 Česká republika, domácí), Eva JANOVA (203 Česká republika), Jarmila OSICKOVA (203 Česká republika), Mirko VYSKOCIL (203 Česká republika), Marketa SEDLINSKA (203 Česká republika), Ladislav DUŠEK (203 Česká republika, domácí), Eliane MARTI (756 Švýcarsko) a Petr HORIN (203 Česká republika).
Vydání VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, Amsterdam, ELSEVIER SCIENCE BV, 2013, 0165-2427.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30300 3.3 Health sciences
Stát vydavatele Nizozemsko
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 1.748
Kód RIV RIV/00216224:14110/13:00067927
Organizační jednotka Lékařská fakulta
Doi http://dx.doi.org/10.1016/j.vetimm.2012.12.013
UT WoS 000316592200009
Klíčová slova anglicky Insect bite hypersensitivity; Horse; Single nucleotide polymorphism; Immunity-related genes
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Ing. Mgr. Věra Pospíšilíková, učo 9005. Změněno: 9. 8. 2013 16:20.
Anotace
Equine insect bite hypersensitivity (IBH) is a seasonal IgE-mediated dermatosis caused by bites of insects of the genus Culicoides. A familial predisposition for the disease has been shown but, except for the MHC, the genes involved have not been identified so far. An immunogenomic analysis of IBH was performed in a model population of Old Kladruby horses, all living in the same environment. Clinical signs of IBH were used as phenotypic manifestation of IBH. Furthermore, total serum IgE levels were determined in the sera of these horses and used as an independent phenotypic marker for the immunogenetic analysis. Single nucleotide polymorphisms (SNPs) in candidate immunity-related genes were used for association analyses. Genotypes composed of two to five genes encoding interferon gamma – IFNG, transforming growth factor beta 1 – TGFB1, Janus kinase 2 – JAK2, thymic stromal lymphopoietin – TSLP, and involucrin – IVL were associated with IBH, indicating a role of the genes in the pathogenesis of IBH. These findings were supported by analysis of gene expression in skin biopsies of 15 affected and 15 unaffected horses. Two markers associated with IBH, IFNG and TGFB1, showed differences in mRNA expression in skin biopsies from IBH-affected and non-affected horses (p < 0.05). Expression of the gene coding for the CD14 receptor molecule – CD14 was different in skin biopsies at p < 0.06. When total IgE levels were treated as binary traits, genotypes of IGHE, ELA-DRA, and IL10/b were associated with this trait. When treated as a continuous trait, total IgE levels were associated with genes IGHE, FCER1A, IL4, IL4R, IL10, IL1RA, and JAK2. This first report on non-MHC genes associated with IBH in horses is thus supported by differences in expression of genes known to play a role in allergy and immunity.
VytisknoutZobrazeno: 16. 8. 2022 22:15