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@article{1092054,
author = {Vychodilova, Leona and Matiasovic, Jan and Bobrova, Olga and Futas, Jan and Klumplerova, Marie and Stejskalova, Karla and Cvanová, Michaela and Janova, Eva and Osickova, Jarmila and Vyskocil, Mirko and Sedlinska, Marketa and Dušek, Ladislav and Marti, Eliane and Horin, Petr},
article_location = {Amsterdam},
article_number = {3-4},
doi = {http://dx.doi.org/10.1016/j.vetimm.2012.12.013},
keywords = {Insect bite hypersensitivity; Horse; Single nucleotide polymorphism; Immunity-related genes},
language = {eng},
issn = {0165-2427},
journal = {VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY},
title = {Immunogenomic analysis of insect bite hypersensitivity in a model horse population},
url = {http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271206&_user=835458&_pii=S0165242712004503&_check=y&_origin=article&_zone=toolbar&_coverDate=2013--15&view=c&originContentFamily=serial&wchp=dGLbVlB-zSkWA&md5=c61f8a0768d38361bac1666e6e71f171&pi},
volume = {152},
year = {2013}
}
TY - JOUR
ID - 1092054
AU - Vychodilova, Leona - Matiasovic, Jan - Bobrova, Olga - Futas, Jan - Klumplerova, Marie - Stejskalova, Karla - Cvanová, Michaela - Janova, Eva - Osickova, Jarmila - Vyskocil, Mirko - Sedlinska, Marketa - Dušek, Ladislav - Marti, Eliane - Horin, Petr
PY - 2013
TI - Immunogenomic analysis of insect bite hypersensitivity in a model horse population
JF - VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
VL - 152
IS - 3-4
SP - 260–268
EP - 260–268
PB - ELSEVIER SCIENCE BV
SN - 01652427
KW - Insect bite hypersensitivity
KW - Horse
KW - Single nucleotide polymorphism
KW - Immunity-related genes
UR - http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271206&_user=835458&_pii=S0165242712004503&_check=y&_origin=article&_zone=toolbar&_coverDate=2013--15&view=c&originContentFamily=serial&wchp=dGLbVlB-zSkWA&md5=c61f8a0768d38361bac1666e6e71f171&pi
N2 - Equine insect bite hypersensitivity (IBH) is a seasonal IgE-mediated dermatosis caused by bites of insects of the genus Culicoides. A familial predisposition for the disease has been shown but, except for the MHC, the genes involved have not been identified so far. An immunogenomic analysis of IBH was performed in a model population of Old Kladruby horses, all living in the same environment. Clinical signs of IBH were used as phenotypic manifestation of IBH. Furthermore, total serum IgE levels were determined in the sera of these horses and used as an independent phenotypic marker for the immunogenetic analysis. Single nucleotide polymorphisms (SNPs) in candidate immunity-related genes were used for association analyses. Genotypes composed of two to five genes encoding interferon gamma – IFNG, transforming growth factor beta 1 – TGFB1, Janus kinase 2 – JAK2, thymic stromal lymphopoietin – TSLP, and involucrin – IVL were associated with IBH, indicating a role of the genes in the pathogenesis of IBH. These findings were supported by analysis of gene expression in skin biopsies of 15 affected and 15 unaffected horses. Two markers associated with IBH, IFNG and TGFB1, showed differences in mRNA expression in skin biopsies from IBH-affected and non-affected horses (p < 0.05). Expression of the gene coding for the CD14 receptor molecule – CD14 was different in skin biopsies at p < 0.06. When total IgE levels were treated as binary traits, genotypes of IGHE, ELA-DRA, and IL10/b were associated with this trait. When treated as a continuous trait, total IgE levels were associated with genes IGHE, FCER1A, IL4, IL4R, IL10, IL1RA, and JAK2. This first report on non-MHC genes associated with IBH in horses is thus supported by differences in expression of genes known to play a role in allergy and immunity.
ER -
VYCHODILOVA, Leona, Jan MATIASOVIC, Olga BOBROVA, Jan FUTAS, Marie KLUMPLEROVA, Karla STEJSKALOVA, Michaela CVANOVÁ, Eva JANOVA, Jarmila OSICKOVA, Mirko VYSKOCIL, Marketa SEDLINSKA, Ladislav DUŠEK, Eliane MARTI a Petr HORIN. Immunogenomic analysis of insect bite hypersensitivity in a model horse population. \textit{VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY}. Amsterdam: ELSEVIER SCIENCE BV, 2013, roč.~152, 3-4, s.~260–268. ISSN~0165-2427. doi:10.1016/j.vetimm.2012.12.013.
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