Detailed Information on Publication Record
2012
Engineered recombinant enzyme haloalkane dehalogenase DhaA85
CHALOUPKOVÁ, Radka, Zbyněk PROKOP, Jiří DAMBORSKÝ and Táňa KOUDELÁKOVÁBasic information
Original name
Engineered recombinant enzyme haloalkane dehalogenase DhaA85
Authors
CHALOUPKOVÁ, Radka (203 Czech Republic, belonging to the institution), Zbyněk PROKOP (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Táňa KOUDELÁKOVÁ (203 Czech Republic)
Edition
2012
Other information
Language
English
Type of outcome
Technicky realizované výsledky (prototyp, funkční vzorek)
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/12:00064441
Organization unit
Faculty of Science
Keywords in English
haloalkane dehalogenase; DhaA85
Technical parameters
výrobní postup, funkční vzorek byl vyroben na základě ověření vlastností, licenční smlouva nebyla zatím uzavřena.
Tags
International impact, Reviewed
Změněno: 8/4/2013 12:41, prof. Mgr. Jiří Damborský, Dr.
Abstract
V originále
Thermostable haloalkane dehalogenase DhaA85 prepared by saturated mutagenesis. Mutations targeting as few as four residues lining the access tunnel extended enzyme’s half-life in 40% dimethyl sulfoxide from minutes to weeks (4,000-fold) and increased its melting temperature by 19 C. Protein crystallography and molecular dynamics revealed that the tunnel residue packing is a key determinant of protein stability and the active-site accessibility for co-solvent molecules (red dots). The broad applicability of this concept was verified by analyzing twenty six proteins with buried active sites from all six enzyme classes.The results was made based on the verification of properties.
Links
| ED0001/01/01, research and development project |
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