Podrobný výpis o publikaci

ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, Mariana BENITEZ KEINRAD, Ondrej ŠEDO, David POTĚŠIL, Ondřej NOVÁK, Jana SVAČINOVÁ, Bedřich PEŠEK, Jiří MALBECK, Zbyněk ZDRÁHAL a Jan HEJÁTKO. Proteome regulation in Arabidopsis reveals shoot- and root specific effects of cytokinins on hormonal homeostasis. In Functional Genomics and Proteomics for Sustainable Agriculture, a conference commemorating the 190th birth anniversary of Gregor Mendel. 2012.

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TY  - CONF
ID  - 1092572
AU  - Žďárská, Markéta - Zatloukalová, Pavlína - Benitez Keinrad, Mariana - Šedo, Ondrej - Potěšil, David - Novák, Ondřej - Svačinová, Jana - Pešek, Bedřich - Malbeck, Jiří - Zdráhal, Zbyněk - Hejátko, Jan
PY  - 2012
TI  - Proteome regulation in Arabidopsis reveals shoot- and root specific effects of cytokinins on hormonal homeostasis
KW  - Arabidopsis, proteome, cytokinin, ethylene, crosstalk
N2  - Cytokinins (CK) mediate a large spectrum of developmental and physiological regulations in plants. Shoot and root reveal specific and sometimes even contrary responses to CK. However, the molecular mechanisms underlying the shoot and root specificity of CK-mediated regulations remain mostly unclear. We show that differential CK-mediated proteome regulation underlies the shoot and root specificity of CK response at the hormonal metabolism. Overall, we identified 43/18 differentially regulated proteins in the shoot and 31/21 in the root in the early/delayed response, respectively. We demonstrate that CK predominantly regulate proteins involved in carbohydrate and energy metabolism in the shoot and in protein synthesis and destination in the root. In the root we identified 3 out of 4 enzymes of the ethylene biosynthetic pathway to be upregulated in an early CK response, suggesting that majority of the ethylene biosynthetic pathway is under strong control of CK in the root. On the other hand, in the shoot we identified several abscisic acid (ABA)-related proteins to be upregulated by CK. To substantiate potential crosstalk of BAP with metabolism of other hormones, we analyzed changes of the endogenous hormone levels after CK application. In a good accordance with our proteomic data, we found prompt upregulation of 1-aminocyclopropane-1-carboxylic acid, the rate-limiting precursor of ethylene biosynthesis in the root, while ABA was upregulated in the early response of the shoot. In conclusion, we propose tissue-specific proteome response and CK-mediated hormonal crosstalk as important factors contributing to the long-known specificity of CK regulations over shoot and root development.
ER  -

Základní údaje
Originální název	Proteome regulation in Arabidopsis reveals shoot- and root specific effects of cytokinins on hormonal homeostasis
Autoři	ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, Mariana BENITEZ KEINRAD, Ondrej ŠEDO, David POTĚŠIL, Ondřej NOVÁK, Jana SVAČINOVÁ, Bedřich PEŠEK, Jiří MALBECK, Zbyněk ZDRÁHAL a Jan HEJÁTKO.
Vydání	Functional Genomics and Proteomics for Sustainable Agriculture, a conference commemorating the 190th birth anniversary of Gregor Mendel, 2012.

Další údaje
Originální jazyk	angličtina
Typ výsledku	Konferenční abstrakt
Obor	Genetika a molekulární biologie
Stát vydavatele	Česká republika
Utajení	není předmětem státního či obchodního tajemství
Organizační jednotka	Středoevropský technologický institut
Klíčová slova česky	Arabidopsis, proteom, cytokinin, ethylén, interakce
Klíčová slova anglicky	Arabidopsis, proteome, cytokinin, ethylene, crosstalk
Příznaky	Mezinárodní význam
Změnil	Změnila: Mgr. Markéta Žďárská, Ph.D., učo 107631. Změněno: 3. 4. 2013 10:13.

Anotace
Cytokinins (CK) mediate a large spectrum of developmental and physiological regulations in plants. Shoot and root reveal specific and sometimes even contrary responses to CK. However, the molecular mechanisms underlying the shoot and root specificity of CK-mediated regulations remain mostly unclear. We show that differential CK-mediated proteome regulation underlies the shoot and root specificity of CK response at the hormonal metabolism. Overall, we identified 43/18 differentially regulated proteins in the shoot and 31/21 in the root in the early/delayed response, respectively. We demonstrate that CK predominantly regulate proteins involved in carbohydrate and energy metabolism in the shoot and in protein synthesis and destination in the root. In the root we identified 3 out of 4 enzymes of the ethylene biosynthetic pathway to be upregulated in an early CK response, suggesting that majority of the ethylene biosynthetic pathway is under strong control of CK in the root. On the other hand, in the shoot we identified several abscisic acid (ABA)-related proteins to be upregulated by CK. To substantiate potential crosstalk of BAP with metabolism of other hormones, we analyzed changes of the endogenous hormone levels after CK application. In a good accordance with our proteomic data, we found prompt upregulation of 1-aminocyclopropane-1-carboxylic acid, the rate-limiting precursor of ethylene biosynthesis in the root, while ABA was upregulated in the early response of the shoot. In conclusion, we propose tissue-specific proteome response and CK-mediated hormonal crosstalk as important factors contributing to the long-known specificity of CK regulations over shoot and root development.

Anotace

Cytokinins (CK) mediate a large spectrum of developmental and physiological regulations in plants. Shoot and root reveal specific and sometimes even contrary responses to CK. However, the molecular mechanisms underlying the shoot and root specificity of CK-mediated regulations remain mostly unclear. We show that differential CK-mediated proteome regulation underlies the shoot and root specificity of CK response at the hormonal metabolism. Overall, we identified 43/18 differentially regulated proteins in the shoot and 31/21 in the root in the early/delayed response, respectively. We demonstrate that CK predominantly regulate proteins involved in carbohydrate and energy metabolism in the shoot and in protein synthesis and destination in the root. In the root we identified 3 out of 4 enzymes of the ethylene biosynthetic pathway to be upregulated in an early CK response, suggesting that majority of the ethylene biosynthetic pathway is under strong control of CK in the root. On the other hand, in the shoot we identified several abscisic acid (ABA)-related proteins to be upregulated by CK. To substantiate potential crosstalk of BAP with metabolism of other hormones, we analyzed changes of the endogenous hormone levels after CK application. In a good accordance with our proteomic data, we found prompt upregulation of 1-aminocyclopropane-1-carboxylic acid, the rate-limiting precursor of ethylene biosynthesis in the root, while ABA was upregulated in the early response of the shoot. In conclusion, we propose tissue-specific proteome response and CK-mediated hormonal crosstalk as important factors contributing to the long-known specificity of CK regulations over shoot and root development.

Návaznosti
ED1.1.00/02.0068, projekt VaV	Název: CEITEC - central european institute of technology