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@article{1093267,
author = {Kiran, Nagavalli Subbanna and Benková, Eva and Reková, Alena and Dubová, Jaroslava and Malbeck, Jiří and Palme, Klaus and Brzobohatý, Břetislav},
article_location = {Oxford},
article_number = {06},
doi = {http://dx.doi.org/10.1016/j.phytochem.2012.03.012},
keywords = {Nicotiana tabacum; Tobacco; beta-Glucosidase; Zeatin-O-glucoside; Sub-cellular compartmentation; Cytokinin metabolism},
language = {eng},
issn = {0031-9422},
journal = {Phytochemistry},
title = {Retargeting a maize b-glucosidase to the vacuole – Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole},
url = {http://www.sciencedirect.com/science/article/pii/S0031942212001458},
volume = {79},
year = {2012}
}
TY - JOUR
ID - 1093267
AU - Kiran, Nagavalli Subbanna - Benková, Eva - Reková, Alena - Dubová, Jaroslava - Malbeck, Jiří - Palme, Klaus - Brzobohatý, Břetislav
PY - 2012
TI - Retargeting a maize b-glucosidase to the vacuole – Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole
JF - Phytochemistry
VL - 79
IS - 06
SP - 67-77
EP - 67-77
PB - Pergamon - Elsevier Science Ltd.
SN - 00319422
KW - Nicotiana tabacum
KW - Tobacco
KW - beta-Glucosidase
KW - Zeatin-O-glucoside
KW - Sub-cellular compartmentation
KW - Cytokinin metabolism
UR - http://www.sciencedirect.com/science/article/pii/S0031942212001458
L2 - http://www.sciencedirect.com/science/article/pii/S0031942212001458
N2 - Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determined in part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize b-glucosidase capable of releasing active cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl. The presence of the enzyme in these ectopic structures is shown by histochemical staining. CK quantification reveals that these transgenic seedlings are unable to accumulate zeatin-O-glucoside to levels similar to those observed in the wild type. When crossed with tobacco overexpressing the zeatin- O-glucosyltransferase gene from Phaseolus, the vacuolar variant shows an almost complete reversion in the root elongation assay. This is the first evidence from intact plants that the vacuole is the storage organelle for CK O-glucosides and that they are available to attack by Zm-p60.1. We propose the use of Zm-p60.1 as a robust molecular tool that exploits the reversibility of O-glucosylation and enables delicate manipulations of active CK content at the cellular level.
ER -
KIRAN, Nagavalli Subbanna, Eva BENKOVÁ, Alena REKOVÁ, Jaroslava DUBOVÁ, Jiří MALBECK, Klaus PALME a Břetislav BRZOBOHATÝ. Retargeting a maize b-glucosidase to the vacuole – Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole. \textit{Phytochemistry}. Oxford: Pergamon - Elsevier Science Ltd., 2012, roč.~79, č.~06, s.~67-77. ISSN~0031-9422. Dostupné z: https://dx.doi.org/10.1016/j.phytochem.2012.03.012.
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