Identification of cytokinin receptors of Brassica napus

KUDEROVÁ, Alena, Katarína KURICOVÁ, Eliška NEJEDLÁ, Anna ČURDOVÁ, Lenka MICENKOVÁ, David ŠMAJS and Jan HEJÁTKO. Identification of cytokinin receptors of Brassica napus. In Functional Genomics and Proteomics for Sustainable Agriculture book of abstracts, a conference commemorating the 190th birth anniversary of Gregor Mendel, Brno, 13th-14th June 2012. 2012. ISBN 978-80-7375-624-6.

Basic information

• Original name: Identification of cytokinin receptors of Brassica napus
• Authors: KUDEROVÁ, Alena (203 Czech Republic, belonging to the institution), Katarína KURICOVÁ (703 Slovakia, belonging to the institution), Eliška NEJEDLÁ (203 Czech Republic, belonging to the institution), Anna ČURDOVÁ (703 Slovakia, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), David ŠMAJS (203 Czech Republic, belonging to the institution) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Functional Genomics and Proteomics for Sustainable Agriculture book of abstracts, a conference commemorating the 190th birth anniversary of Gregor Mendel, Brno, 13th-14th June 2012, 2012.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: Functional Genomics and Proteomics for Sustainable Agriculture book of abstracts : a conference commemorating the 190th birth anniversary of Gregor Mendel : Brno, 13th-14th June 2012
• RIV identification code: RIV/00216224:14740/12:00064588
• Organization unit: Central European Institute of Technology
• ISBN: 978-80-7375-624-6
• Keywords in English: plant hormone- multistep His-Asp phosphorelay-cytokinin receptor-CHASE-containing histidine kinase-Arabidopsis-Brassica napus-JBnB library-heterologous hybridization-gene structure-BnCHK

Abstract

Cytokinins (CK) belong to a group of plant hormones, playing crucial role in plant growth and development. CK signal is transduced through a multistep phosphorelay where the phosphoryl group is transferred between His and Asp residues. Cytokinin perception has been well-studied and the CHASE-containing sensor His kinases (CHKs) have been shown to be important morphological factors in Arabidopsis. As Brassica napus shares a high degree of nucleotide identity in coding regions with Arabidopsis, we used sequences of A. thaliana His kinase genes (AHKs) to search for their orthologs in B. napus (BnCHKs). Experimental screen of bacterial artificial chromosome (BAC) genomic DNA library of B. napus, var. Tapidor (JBnB), resulted in identification of redundant number of overlapping BAC clones, which hybridized with PCR-generated probes derived from sequences of phylogenetically conserved AHK regions. Five selected clones have been sequenced and defined as CK receptors by computational analysis. Based on prediction, gene-specific primers have been designed and coding- as well as untranslated cDNA regions cloned. Gene structure analysis and prediction of conserved structural domains of five BnCHK proteins are presented. Four homologues of AHK2 and one homologue of AHK3 have been identified by this experimental approach.

Links

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations