MAŠKA, Martin, Ondřej DANĚK, Saray GARASA, Ana ROUZAUT, Arrate MUÑOZ-BARRUTIA and Carlos ORTIZ-DE-SOLÓRZANO. Segmentation and Shape Tracking of Whole Fluorescent Cells Based on the Chan-Vese Model. IEEE Transactions on Medical Imaging. 2013, vol. 32, No 6, p. 995-1006. ISSN 0278-0062. Available from: https://dx.doi.org/10.1109/TMI.2013.2243463.
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Basic information
Original name Segmentation and Shape Tracking of Whole Fluorescent Cells Based on the Chan-Vese Model
Authors MAŠKA, Martin (203 Czech Republic, guarantor, belonging to the institution), Ondřej DANĚK (203 Czech Republic, belonging to the institution), Saray GARASA (724 Spain), Ana ROUZAUT (724 Spain), Arrate MUÑOZ-BARRUTIA (724 Spain) and Carlos ORTIZ-DE-SOLÓRZANO (724 Spain).
Edition IEEE Transactions on Medical Imaging, 2013, 0278-0062.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.799
RIV identification code RIV/00216224:14330/13:00081861
Organization unit Faculty of Informatics
Doi http://dx.doi.org/10.1109/TMI.2013.2243463
UT WoS 000319701800003
Keywords in English Cell tracking;Chan–Vese model;fluorescence microscopy;graph cut optimization;level set framework
Tags cbia-web
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Martin Maška, Ph.D., učo 60734. Changed: 13/4/2018 14:55.
Abstract
We present a fast and robust approach to tracking the evolving shape of whole fluorescent cells in time-lapse series. The proposed tracking scheme involves two steps. First, coherence-enhancing diffusion filtering is applied on each frame to reduce the amount of noise and enhance flow-like structures. Second, the cell boundaries are detected by minimizing the Chan–Vese model in the fast level set-like and graph cut frameworks. To allow simultaneous tracking of multiple cells over time, both frameworks have been integrated with a topological prior exploiting the object indication function. The potential of the proposed tracking scheme and the advantages and disadvantages of both frameworks are demonstrated on 2-D and 3-D time-lapse series of rat adipose-derived mesenchymal stem cells and human lung squamous cell carcinoma cells, respectively.
Links
EE2.3.30.0009, research and development projectName: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
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