ER-localized auxin transporter PIN8 regulates auxin homeostasis
and male gametophyte development in Arabidopsis

J 2012

ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

DING, Zhaojun, Bangjun WANG, Ignacio MORENO, Nikoleta DUPLAKOVA, Sibu SIMON et. al.

Basic information

Original name

ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

Authors

DING, Zhaojun (156 China), Bangjun WANG (756 Switzerland), Ignacio MORENO (152 Chile), Nikoleta DUPLAKOVA (203 Czech Republic), Sibu SIMON (56 Belgium), Nicola CARRARO (840 United States of America), Jesica REEMMER (840 United States of America), Ales PENCIK (203 Czech Republic), Xu CHEN (56 Belgium), Ricardo TEJOS (56 Belgium), Petr SKUPA (203 Czech Republic), Stephan POLLMANN (276 Germany), Jozef MRAVEC (56 Belgium), Jan PETRASEK (203 Czech Republic), Eva ZAŽÍMALOVÁ (203 Czech Republic), David HONYS (203 Czech Republic), Jakub ROLCIK (203 Czech Republic), Angus MURPHY (840 United States of America), Ariel ORELLANA (152 Chile), Markus GEISLER (756 Switzerland) and Jiří FRIML (203 Czech Republic, guarantor, belonging to the institution)

Edition

NATURE COMMUNICATIONS, LONDON, NATURE PUBLISHING GROUP, 2012, 2041-1723

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.015

RIV identification code

RIV/00216224:14740/12:00064713

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/ncomms1941

UT WoS

000306995000013

Keywords in English

PLANT DEVELOPMENT; ENDOPLASMIC-RETICULUM; POLLEN GERMINATION; UDP-GLUCOSE; THALIANA; EFFLUX; BIOSYNTHESIS; EXPRESSION; GRADIENTS; MEMBRANE

Abstract

V originále

Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0043, research and development project

Name: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů

LC06034, research and development project

Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

Citovat

DING, Zhaojun, Bangjun WANG, Ignacio MORENO, Nikoleta DUPLAKOVA, Sibu SIMON, Nicola CARRARO, Jesica REEMMER, Ales PENCIK, Xu CHEN, Ricardo TEJOS, Petr SKUPA, Stephan POLLMANN, Jozef MRAVEC, Jan PETRASEK, Eva ZAŽÍMALOVÁ, David HONYS, Jakub ROLCIK, Angus MURPHY, Ariel ORELLANA, Markus GEISLER and Jiří FRIML. ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. NATURE COMMUNICATIONS. LONDON: NATURE PUBLISHING GROUP, 2012, vol. 3, July, p. "nestránkováno", 9 pp. ISSN 2041-1723. Available from: https://dx.doi.org/10.1038/ncomms1941.

@article{1095768,
   author = {Ding, Zhaojun and Wang, Bangjun and Moreno, Ignacio and Duplakova, Nikoleta and Simon, Sibu and Carraro, Nicola and Reemmer, Jesica and Pencik, Ales and Chen, Xu and Tejos, Ricardo and Skupa, Petr and Pollmann, Stephan and Mravec, Jozef and Petrasek, Jan and Zažímalová, Eva and Honys, David and Rolcik, Jakub and Murphy, Angus and Orellana, Ariel and Geisler, Markus and Friml, Jiří},
   article_location = {LONDON},
   article_number = {July},
   doi = {http://dx.doi.org/10.1038/ncomms1941},
   keywords = {PLANT DEVELOPMENT; ENDOPLASMIC-RETICULUM; POLLEN GERMINATION; UDP-GLUCOSE; THALIANA; EFFLUX; BIOSYNTHESIS; EXPRESSION; GRADIENTS; MEMBRANE},
   language = {eng},
   issn = {2041-1723},
   journal = {NATURE COMMUNICATIONS},
   title = {ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/22760640},
   volume = {3},
   year = {2012}
}

TY  - JOUR
ID  - 1095768
AU  - Ding, Zhaojun - Wang, Bangjun - Moreno, Ignacio - Duplakova, Nikoleta - Simon, Sibu - Carraro, Nicola - Reemmer, Jesica - Pencik, Ales - Chen, Xu - Tejos, Ricardo - Skupa, Petr - Pollmann, Stephan - Mravec, Jozef - Petrasek, Jan - Zažímalová, Eva - Honys, David - Rolcik, Jakub - Murphy, Angus - Orellana, Ariel - Geisler, Markus - Friml, Jiří
PY  - 2012
TI  - ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis
JF  - NATURE COMMUNICATIONS
VL  - 3
IS  - July
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - NATURE PUBLISHING GROUP
SN  - 20411723
KW  - PLANT DEVELOPMENT
KW  - ENDOPLASMIC-RETICULUM
KW  - POLLEN GERMINATION
KW  - UDP-GLUCOSE
KW  - THALIANA
KW  - EFFLUX
KW  - BIOSYNTHESIS
KW  - EXPRESSION
KW  - GRADIENTS
KW  - MEMBRANE
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22760640
L2  - http://www.ncbi.nlm.nih.gov/pubmed/22760640
N2  - Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.
ER  -

DING, Zhaojun, Bangjun WANG, Ignacio MORENO, Nikoleta DUPLAKOVA, Sibu SIMON, Nicola CARRARO, Jesica REEMMER, Ales PENCIK, Xu CHEN, Ricardo TEJOS, Petr SKUPA, Stephan POLLMANN, Jozef MRAVEC, Jan PETRASEK, Eva ZAŽÍMALOVÁ, David HONYS, Jakub ROLCIK, Angus MURPHY, Ariel ORELLANA, Markus GEISLER and Jiří FRIML. ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis. \textit{NATURE COMMUNICATIONS}. LONDON: NATURE PUBLISHING GROUP, 2012, vol.~3, July, p.~''nestránkováno'', 9 pp. ISSN~2041-1723. Available from: https://dx.doi.org/10.1038/ncomms1941.

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