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@article{1102512,
author = {Ručka, Zdeněk and Vaňhara, Petr and Krontorád Koutná, Irena and Tesařová, Lenka and Potěšilová, Michaela and Stejskal, Stanislav and Šimara, Pavel and Doležel, Jan and Zvoníček, Václav and Coufal, Oldřich and Čapov, Ivan},
article_location = {UNITED STATES},
article_number = {1},
doi = {http://dx.doi.org/10.3892/ijmm.2013.1363},
keywords = {insulin; dexamethasone; surfactant; lung carcinoma; A549; H441},
language = {eng},
issn = {1107-3756},
journal = {INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE},
title = {Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23620014},
volume = {32},
year = {2013}
}
TY - JOUR
ID - 1102512
AU - Ručka, Zdeněk - Vaňhara, Petr - Krontorád Koutná, Irena - Tesařová, Lenka - Potěšilová, Michaela - Stejskal, Stanislav - Šimara, Pavel - Doležel, Jan - Zvoníček, Václav - Coufal, Oldřich - Čapov, Ivan
PY - 2013
TI - Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue
JF - INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
VL - 32
IS - 1
SP - 211-218
EP - 211-218
PB - SPANDIDOS PUBL LTD
SN - 11073756
KW - insulin
KW - dexamethasone
KW - surfactant
KW - lung carcinoma
KW - A549
KW - H441
UR - http://www.ncbi.nlm.nih.gov/pubmed/23620014
L2 - http://www.ncbi.nlm.nih.gov/pubmed/23620014
N2 - In this study, the effects of insulin and dexamethasone on the expression and mRNA transcription of 4 pulmonary surfactant-associated proteins [surfactant protein (SFTP)A, SFTPB, SFTPC and SFTPD] were examined. The commercially available cell lines, A549 and H441, were used as acceptable models of lung surfactant-producing cells. Subsequently, the effects of insulin on the expression of surfactant-associated proteins were examined in patients with lung adenocarcinoma during lung resection. Our results demonstrated the inhibitory effects of insulin on the transcription of the SFTPB, SFTPC and SFTPD genes in H441 cells and the SFTPB gene in A549 cells. Treatment with insulin significantly decreased the protein expression of SFTPA1 and SFTPA2 in the H441 cells and that of proSFTPB in the A549 cells. Dexamethasone promoted the transcription of the SFTPB, SFTPC and SFTPD genes in the A549 and H441 cells and reduced the transcription of the SFTPA1 and SFTPA2 genes in the H441 cells (SFTPA mRNA expression was not detected in A549 cells). Furthermore, we demonstrated that the mRNA levels of the selected genes were significantly lower in the cell lines compared to the lung tissue. A549 and H441 cells represent similar cell types. Yet, in our experiments, these cells reacted differently to insulin and/or dexamethasone treatment, and the mRNA levels of their main protein products, surfactant-associated proteins,were significantly lower than those in real tissue. Therefore, the results obtained in this study challenge the suitability of A549 and H441 cells as models of type II pneumocytes and Clara cells, respectively. However, we successfully demonstrate the possibility of studying the effects of insulin on pulmonary surfactant-associated genes and proteins in patients with lung adenocarcinoma.
ER -
RUČKA, Zdeněk, Petr VAŇHARA, Irena KRONTORÁD KOUTNÁ, Lenka TESAŘOVÁ, Michaela POTĚŠILOVÁ, Stanislav STEJSKAL, Pavel ŠIMARA, Jan DOLEŽEL, Václav ZVONÍČEK, Oldřich COUFAL and Ivan ČAPOV. Differential effects of insulin and dexamethasone on pulmonary surfactant-associated genes and proteins in A549 and H441 cells and lung tissue. \textit{INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE}. UNITED STATES: SPANDIDOS PUBL LTD, 2013, vol.~32, No~1, p.~211-218. ISSN~1107-3756. Available from: https://dx.doi.org/10.3892/ijmm.2013.1363.
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