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@article{1102518, author = {Faltejsková, Petra and Bočánek, Ondřej and Šachlová, Milana and Svoboda, Marek and Kiss, Igor and Vyzula, Rostislav and Slabý, Ondřej}, article_location = {Amsterdam}, article_number = {4-5}, doi = {http://dx.doi.org/10.3233/CBM-130308}, keywords = {microRNAs; serum; colorectal cancer; biomarkers}, language = {eng}, issn = {1574-0153}, journal = {Cancer Biomarkers}, title = {Circulating miR-17-3p, miR-29a, miR-92a and miR-135b in serum: Evidence against their usage as biomarkers in colorectal cancer}, volume = {12}, year = {2012} }
TY - JOUR ID - 1102518 AU - Faltejsková, Petra - Bočánek, Ondřej - Šachlová, Milana - Svoboda, Marek - Kiss, Igor - Vyzula, Rostislav - Slabý, Ondřej PY - 2012 TI - Circulating miR-17-3p, miR-29a, miR-92a and miR-135b in serum: Evidence against their usage as biomarkers in colorectal cancer JF - Cancer Biomarkers VL - 12 IS - 4-5 SP - 199-204 EP - 199-204 PB - IOS Press SN - 15740153 KW - microRNAs KW - serum KW - colorectal cancer KW - biomarkers N2 - BACKGROUND: Colorectal cancer (CRC) is one of the leading causes of cancer-related death in the world. Therefore, there is a high demand for cost-effective and non-invasive biomarkers that would enable an early detection of asymptomatic and curable disease with high sensitivity and specificity. OBJECTIVE: The main objective of this study was to investigate the potential of circulating miRNAs as biomarkers of CRC. METHODS: Total RNA enriched for small RNAs was isolated from 100~sera of patients with CRC and 30 sera of healthy donors. The expression levels of miR-17-3p, miR-29a, miR-92a and miR-135b were determined using quantitative real-time PCR. The average expression levels of particular miRNAs were normalized to miR-16 levels and statistically evaluated. RESULTS: Using Mann-Whitney U test, no significant differences were observed in miR-17-3p (P=0.18), miR-29a (P=0.14) and miR-92a (P=0.60) levels between sera of CRC patients and controls. The levels of miR-135b in serum were too low to be quantified accurately. Subsequently, we tried to correlate expression levels of analyzed miRNAs to clinical-pathological features of CRC patients. Only levels of mir-29a were correlated with the clinical stage (P=0.04). Expression levels of the other miRNAs were correlated neither with the clinical stage, nor with the grade. CONCLUSIONS: Interestingly, our results are contradictory to previous studies performed on the CRC patients from Chinese population, providing an evidence against usage of serum miR-17-3p, miR-29a, miR-92a and miR-135b as new biomarkers for early detection of CRC. ER -
FALTEJSKOVÁ, Petra, Ondřej BOČÁNEK, Milana ŠACHLOVÁ, Marek SVOBODA, Igor KISS, Rostislav VYZULA a Ondřej SLABÝ. Circulating miR-17-3p, miR-29a, miR-92a and miR-135b in serum: Evidence against their usage as biomarkers in colorectal cancer. \textit{Cancer Biomarkers}. Amsterdam: IOS Press, 2012, roč.~12, 4-5, s.~199-204. ISSN~1574-0153. Dostupné z: https://dx.doi.org/10.3233/CBM-130308.
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