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@article{1102521,
author = {Slabý, Ondřej and Šachlová, Milana and Březková, Veronika and Héžová, Renata and Kovaříková, Alena and Bischofová, Svatava and Ševčíková, Sabina and Bienertová Vašků, Julie and Vašků, Anna and Svoboda, Marek and Vyzula, Rostislav},
article_location = {Abingdon},
article_number = {2},
doi = {http://dx.doi.org/10.1080/01635581.2013.756530},
keywords = {microRNAs; colorectal cancer; isothiocyanates; polymorphism},
language = {eng},
issn = {0163-5581},
journal = {Nutrition and cancer : an international journal},
title = {Identification of MicroRNAs Regulated by Isothiocyanates and Association of Polymorphisms Inside Their Target Sites with Risk of Sporadic Colorectal Cancer},
volume = {65},
year = {2013}
}
TY - JOUR
ID - 1102521
AU - Slabý, Ondřej - Šachlová, Milana - Březková, Veronika - Héžová, Renata - Kovaříková, Alena - Bischofová, Svatava - Ševčíková, Sabina - Bienertová Vašků, Julie - Vašků, Anna - Svoboda, Marek - Vyzula, Rostislav
PY - 2013
TI - Identification of MicroRNAs Regulated by Isothiocyanates and Association of Polymorphisms Inside Their Target Sites with Risk of Sporadic Colorectal Cancer
JF - Nutrition and cancer : an international journal
VL - 65
IS - 2
SP - 247-254
EP - 247-254
PB - Routledge Journals, Taylor & Francis
SN - 01635581
KW - microRNAs
KW - colorectal cancer
KW - isothiocyanates
KW - polymorphism
N2 - Sporadic colorectal cancer (CRC) is a typical multifactorial disease. Isothiocyanates (ITC) have been recently shown to inhibit development of CRC in many experimental models. MicroRNAs (miRNAs) are short noncoding RNAs that posttranscriptionally regulate gene expression through binding to 3 untranslated regions of target mRNAs. MiRNAs are regulated by natural agents, ITCs included. In our study, using global expression profiling based on TaqMan Low-Density Arrays, we identified 3 common miRNAs (miR-155, miR-23b, miR-27b) regulated by ITCs (sulforaphane, iberin) in colonic epithelial cell lines NCM460 and NCM356. In silico predictions allowed us to find 9 relevant single nucleotide polymorphisms (SNPs) localized within the 3UTRs of genes (AGTR1, TNFAIP2, PRKCB, HSPA9, RABGAP1, DICER1, ADAM19, VWA5A, and SIRT5) targeted by these ITC-related miRNAs. Finally, we observed that homozygous CC genotype of DICER1, rs1057035, was significantly associated with decreased risk of CRC (odds ratio = 0.49; 95% confidence interval: 0.25–0.95, P = 0.036) when compared to TT homozygote genotype; also, the C allele tended to have a protective effect (P = 0.072). This study showed that miRNAs could be involved in chemoprotective effects of natural agents; their function alteration through SNPs in their binding sites and flanking regions presents a new class of CRC risk factors.
ER -
SLABÝ, Ondřej, Milana ŠACHLOVÁ, Veronika BŘEZKOVÁ, Renata HÉŽOVÁ, Alena KOVAŘÍKOVÁ, Svatava BISCHOFOVÁ, Sabina ŠEVČÍKOVÁ, Julie BIENERTOVÁ VAŠKŮ, Anna VAŠKŮ, Marek SVOBODA and Rostislav VYZULA. Identification of MicroRNAs Regulated by Isothiocyanates and Association of Polymorphisms Inside Their Target Sites with Risk of Sporadic Colorectal Cancer. \textit{Nutrition and cancer : an international journal}. Abingdon: Routledge Journals, Taylor \&{} Francis, 2013, vol.~65, No~2, p.~247-254. ISSN~0163-5581. Available from: https://dx.doi.org/10.1080/01635581.2013.756530.
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