| HERNYCHOVÁ, Lenka, Marta NEKULOVÁ, David POTĚŠIL, Eva MICHALOVÁ, Zbyněk ZDRÁHAL, Bořivoj VOJTĚŠEK and Jitka HOLČÁKOVÁ. A combined immunoprecipitation and mass spectrometric approach to determine deltaNp63-interacting partners. Klinická onkologie. vol. 25, supplementum 2, p. "2S64"-"2S69", 6 pp. ISSN 0862-495X. 2012. |
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@article{1102821,
author = {Hernychová, Lenka and Nekulová, Marta and Potěšil, David and Michalová, Eva and Zdráhal, Zbyněk and Vojtěšek, Bořivoj and Holčáková, Jitka},
article_number = {supplementum 2},
keywords = {antibodies; p63; immunoprecipitation; proteomics; mass spectrometry; protein; protein interactions},
language = {eng},
issn = {0862-495X},
journal = {Klinická onkologie},
title = {A combined immunoprecipitation and mass spectrometric approach to determine deltaNp63-interacting partners},
url = {http://www.linkos.cz/files/klinicka-onkologie/174/4133.pdf},
volume = {25},
year = {2012}
}
TY - JOUR
ID - 1102821
AU - Hernychová, Lenka - Nekulová, Marta - Potěšil, David - Michalová, Eva - Zdráhal, Zbyněk - Vojtěšek, Bořivoj - Holčáková, Jitka
PY - 2012
TI - A combined immunoprecipitation and mass spectrometric approach to determine deltaNp63-interacting partners
JF - Klinická onkologie
VL - 25
IS - supplementum 2
SP - "2S64"-"2S69"
EP - "2S64"-"2S69"
SN - 0862495X
KW - antibodies
KW - p63
KW - immunoprecipitation
KW - proteomics
KW - mass spectrometry
KW - protein
KW - protein interactions
UR - http://www.linkos.cz/files/klinicka-onkologie/174/4133.pdf
L2 - http://www.linkos.cz/files/klinicka-onkologie/174/4133.pdf
N2 - Expression of p63 is essential for the formation of epidermis and other stratifying epitheliA. Moreover p63 is highly expressed in several epithelial cancers and is involved in tumourigenesis and controlling chemosensitivity. The identification of p63 interacting partners is essential for understanding the complex network of gene regulation managing epithelial development and could also help to reveal signalling pathways participating in UV damage response in human skin. We used a proteomic approach to identify proteins that interact with deltaNp63. Proteins were isolated by immunoprecipitation with deltaNp63 specific antibody and analysed by mass spectrometry. We identified 23 proteins as potential deltaNp63 binding partners that were not present in negative control samples. These results will be evaluated using other methods.
ER -
HERNYCHOVÁ, Lenka, Marta NEKULOVÁ, David POTĚŠIL, Eva MICHALOVÁ, Zbyněk ZDRÁHAL, Bořivoj VOJTĚŠEK and Jitka HOLČÁKOVÁ. A combined immunoprecipitation and mass spectrometric approach to determine deltaNp63-interacting partners. \textit{Klinická onkologie}. vol.~25, supplementum 2, p.~''2S64''-''2S69'', 6 pp. ISSN~0862-495X. 2012.
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