Detailed Information on Publication Record
2013
Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis
KUČERA, Jiří, Pavel BOUCHAL, Jan LOCHMAN, David POTĚŠIL, Oldřich JANICZEK et. al.Basic information
Original name
Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis
Name in Czech
Oxidace železa(II) síru oxidující bakterií Acidithiobacillus ferrooxidans a analýza procesu na úrovni transkripce a syntézy proteinů
Authors
KUČERA, Jiří (203 Czech Republic, belonging to the institution), Pavel BOUCHAL (203 Czech Republic, belonging to the institution), Jan LOCHMAN (203 Czech Republic, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Oldřich JANICZEK (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Martin MANDL (203 Czech Republic, guarantor, belonging to the institution)
Edition
Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, Dordrecht, Springer, 2013, 0003-6072
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.137
RIV identification code
RIV/00216224:14310/13:00066107
Organization unit
Faculty of Science
UT WoS
000316145900021
Keywords (in Czech)
Acidithiobacillus ferrooxidans; oxidace síry, oxidace železa(II); oxidace pyritu; proteomika, RT PCR
Keywords in English
Acidithiobacillus ferrooxidans; elemental sulfur oxidation; ferrous iron oxidation; pyrite oxidation; proteomics; reverse-transcription quantitative PCR
Tags
International impact, Reviewed
Změněno: 13/1/2016 14:26, doc. Ing. Martin Mandl, CSc.
V originále
In contrast to iron-oxidizing Acidithiobacillus ferrooxidans, A. ferrooxidans from a stationary phase elemental sulfur-oxidizing culture exhibited a lag phase in pyrite oxidation, which is similar to its behavior during ferrous iron oxidation. The ability of elemental sulfur-oxidizing A. ferrooxidans to immediately oxidize ferrous iron or pyrite without a lag phase was only observed in bacteria obtained from growing cultures with elemental sulfur. However, these cultures that shifted to ferrous iron oxidation showed a low rate of ferrous iron oxidation while no growth was observed. Two-dimensional gel electrophoresis was used for a quantitative proteomic analysis of the adaptation process when bacteria were switched from elemental sulfur to ferrous iron. A comparison of total cell lysates revealed 39 proteins whose increase or decrease in abundance was related to this phenotypic switching. However, only a few proteins were closely related to iron and sulfur metabolism. Reverse-transcription quantitative PCR was used to further characterize the bacterial adaptation process. The expression profiles of selected genes primarily involved in the ferrous iron oxidation indicated that phenotypic switching is a complex process that includes the activation of genes encoding a membrane protein, maturation proteins, electron transport proteins and their regulators.
In Czech
Ve srovnání se železo oxidující bakterií A. ferrooxidans vykazovala síru oxidující bakterie A. ferrooxidans při oxidaci železa(II) a pyritu odlišné biochemické parametry. Adaptace na oxidaci železa(II) byla monitorována kinetickou, proteomickou a transkriptomickou analýzou za účelem diskuse regulace adaptace.
Links
| CZ.1.05/1.1.00/02.0068, interní kód MU |
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| GA525/08/0697, research and development project |
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