| KUČERA, Jiří, Pavel BOUCHAL, Jan LOCHMAN, David POTĚŠIL, Oldřich JANICZEK, Zbyněk ZDRÁHAL and Martin MANDL. Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Dordrecht: Springer, 2013, vol. 103, No 4, p. 905-919. ISSN 0003-6072. Available from: https://dx.doi.org/10.1007/s10482-012-9872-2. |
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@article{1104551,
author = {Kučera, Jiří and Bouchal, Pavel and Lochman, Jan and Potěšil, David and Janiczek, Oldřich and Zdráhal, Zbyněk and Mandl, Martin},
article_location = {Dordrecht},
article_number = {4},
doi = {http://dx.doi.org/10.1007/s10482-012-9872-2},
keywords = {Acidithiobacillus ferrooxidans; elemental sulfur oxidation; ferrous iron oxidation; pyrite oxidation; proteomics; reverse-transcription quantitative PCR},
language = {eng},
issn = {0003-6072},
journal = {Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology},
title = {Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis},
volume = {103},
year = {2013}
}
TY - JOUR
ID - 1104551
AU - Kučera, Jiří - Bouchal, Pavel - Lochman, Jan - Potěšil, David - Janiczek, Oldřich - Zdráhal, Zbyněk - Mandl, Martin
PY - 2013
TI - Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis
JF - Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
VL - 103
IS - 4
SP - 905-919
EP - 905-919
PB - Springer
SN - 00036072
KW - Acidithiobacillus ferrooxidans
KW - elemental sulfur oxidation
KW - ferrous iron oxidation
KW - pyrite oxidation
KW - proteomics
KW - reverse-transcription quantitative PCR
N2 - In contrast to iron-oxidizing Acidithiobacillus ferrooxidans, A. ferrooxidans from a stationary phase elemental sulfur-oxidizing culture exhibited a lag phase in pyrite oxidation, which is similar to its behavior during ferrous iron oxidation. The ability of elemental sulfur-oxidizing A. ferrooxidans to immediately oxidize ferrous iron or pyrite without a lag phase was only observed in bacteria obtained from growing cultures with elemental sulfur. However, these cultures that shifted to ferrous iron oxidation showed a low rate of ferrous iron oxidation while no growth was observed. Two-dimensional gel electrophoresis was used for a quantitative proteomic analysis of the adaptation process when bacteria were switched from elemental sulfur to ferrous iron. A comparison of total cell lysates revealed 39 proteins whose increase or decrease in abundance was related to this phenotypic switching. However, only a few proteins were closely related to iron and sulfur metabolism. Reverse-transcription quantitative PCR was used to further characterize the bacterial adaptation process. The expression profiles of selected genes primarily involved in the ferrous iron oxidation indicated that phenotypic switching is a complex process that includes the activation of genes encoding a membrane protein, maturation proteins, electron transport proteins and their regulators.
ER -
KUČERA, Jiří, Pavel BOUCHAL, Jan LOCHMAN, David POTĚŠIL, Oldřich JANICZEK, Zbyněk ZDRÁHAL and Martin MANDL. Ferrous iron oxidation by sulfur-oxidizing Acidithiobacillus ferrooxidans and analysis of the process at the levels of transcription and protein synthesis. \textit{Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology}. Dordrecht: Springer, 2013, vol.~103, No~4, p.~905-919. ISSN~0003-6072. Available from: https://dx.doi.org/10.1007/s10482-012-9872-2.
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