| ŠMARDOVÁ, Jana, Květoslava LIŠKOVÁ, Barbora RAVČUKOVÁ, Lenka KUBICZKOVÁ, Sabina ŠEVČÍKOVÁ, Jaroslav MICHÁLEK, Miluše SVITÁKOVÁ, Václav VYBÍHAL, Leoš KŘEN and Jan ŠMARDA. High Frequency of Temperature-Sensitive Mutants of p53 in Glioblastoma. PATHOLOGY & ONCOLOGY RESEARCH. DORDRECHT (Netherlands): Springer, 2013, vol. 19, No 3, p. 421-428. ISSN 1219-4956. Available from: https://dx.doi.org/10.1007/s12253-012-9596-7. |
Other formats:
BibTeX
LaTeX
RIS
@article{1104691,
author = {Šmardová, Jana and Lišková, Květoslava and Ravčuková, Barbora and Kubiczková, Lenka and Ševčíková, Sabina and Michálek, Jaroslav and Svitáková, Miluše and Vybíhal, Václav and Křen, Leoš and Šmarda, Jan},
article_location = {DORDRECHT (Netherlands)},
article_number = {3},
doi = {http://dx.doi.org/10.1007/s12253-012-9596-7},
keywords = {Glioblastoma; p53 tumor suppressor; FASAY; Temperature-sensitive mutation; p53 gene deletion},
language = {eng},
issn = {1219-4956},
journal = {PATHOLOGY & ONCOLOGY RESEARCH},
title = {High Frequency of Temperature-Sensitive Mutants of p53 in Glioblastoma},
volume = {19},
year = {2013}
}
TY - JOUR
ID - 1104691
AU - Šmardová, Jana - Lišková, Květoslava - Ravčuková, Barbora - Kubiczková, Lenka - Ševčíková, Sabina - Michálek, Jaroslav - Svitáková, Miluše - Vybíhal, Václav - Křen, Leoš - Šmarda, Jan
PY - 2013
TI - High Frequency of Temperature-Sensitive Mutants of p53 in Glioblastoma
JF - PATHOLOGY & ONCOLOGY RESEARCH
VL - 19
IS - 3
SP - 421-428
EP - 421-428
PB - Springer
SN - 12194956
KW - Glioblastoma
KW - p53 tumor suppressor
KW - FASAY
KW - Temperature-sensitive mutation
KW - p53 gene deletion
N2 - Glioblastoma is the most common and the most aggressive type of brain cancer. Aberrations of the RTK/RAS/PI3K-, p53-, and RB cell signaling pathways were recognized as a core requirement for pathogenesis of glioblastoma. The p53 tumor suppressor functions as a transcription factor transactivating expression of its target genes in response to various stress stimuli. We determined the p53 status in 36 samples of glioblastoma by functional analyses FASAY and split assay. Seventeen p53 mutations were detected and further analyzed by cDNA and gDNA sequencing in 17 patients (47.2 %). Fifteen (88.2 %) of the mutations were missense mutations causing amino acid substitutions, seven of them exhibited temperature-sensitivity. Two mutations were determined as short deletions, one of them causing formation of premature termination codon in position 247. Fluorescent in situ hybridization revealed the loss of the p53-specific 17p13.3 locus in four of 33 analyzed samples (12 %). In 12 out of 30 samples (40 %), the p53 protein accumulation was shown by immunoblotting. There was high (80 %) concordance between the presence of the clonal p53 mutation and the p53 protein accumulation.
ER -
ŠMARDOVÁ, Jana, Květoslava LIŠKOVÁ, Barbora RAVČUKOVÁ, Lenka KUBICZKOVÁ, Sabina ŠEVČÍKOVÁ, Jaroslav MICHÁLEK, Miluše SVITÁKOVÁ, Václav VYBÍHAL, Leoš KŘEN and Jan ŠMARDA. High Frequency of Temperature-Sensitive Mutants of p53 in Glioblastoma. \textit{PATHOLOGY \&{} ONCOLOGY RESEARCH}. DORDRECHT (Netherlands): Springer, 2013, vol.~19, No~3, p.~421-428. ISSN~1219-4956. Available from: https://dx.doi.org/10.1007/s12253-012-9596-7.
|
