Classification of Aeromonas veronii bv. sobria strains isolated
from human clinical material

a 2013

Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material

ČERNOHLÁVKOVÁ, Jitka, Michaela SEDLÁČKOVÁ, Eva KREJČÍ and Ivo SEDLÁČEK

Basic information

Original name

Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material

Name in Czech

Klasifikace kmenů Aeromonas veronii bv. sobria izolovaných z humánního klinického materiálu

Authors

ČERNOHLÁVKOVÁ, Jitka (203 Czech Republic, guarantor, belonging to the institution), Michaela SEDLÁČKOVÁ (203 Czech Republic), Eva KREJČÍ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic)

Edition

ECCMID 2013, Berlin, Germany, 2013

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/13:00068202

Organization unit

Faculty of Science

Keywords (in Czech)

aeromonády; klasifikace; biotypizace; ribotypizace; sekvencování

Keywords in English

Aeromonads; classification; biotyping; ribotyping; gene sequencing

Abstract

V originále

Aeromonas are gram-negative, oxidase positive, fermenting rods widely distributed in the environment. This genus was recognised as an important fish pathogen, however is often associated with gastrointestinal and extraintestinal diseases in human. Despite its clinical significance Aeromonas spp. are not accurately identified during routine clinical examination, because of their phenotypical similarity. We focused on detailed characterisation of 22 clinical isolates of “A. sobria complex” from diarrhoea samples. Biochemical tests were used to classify isolates into “A. sobria complex”. Further, an approach using ribotyping, whole-cell protein analysis and cpn60 gene sequencing were applied for detailed characterisation and identification. Additionally, 11 reference type strains of Aeromonas from Czech Collection of Microorganisms were included in the comparison. Genetic analysis based on ribotyping clearly distinguished the 22 human isolates from reference Aeromonas type strains and clustered them together with A. veronii bv. sobria, A. veronii bv. veronii and A. allosaccharophila reference strains. A. jandaei and A. schubertii were the next closest relation species. Six isolates were clustered into a separate subclaster(similarity 52%), but results of cpn60 gene sequencing of a selected isolate confirmed A. veronii bv. sobria identification. Whole-cell protein analysis showed different distribution of isolates. 16 isolates were grouped together with A. veronii bv. sobria reference strain (similarity level 48 %) and 8 isolates together with A. veronii bv. veronii reference strain. Other isolates were separated from these groups. Despite inconsistent results of ribotying and whole-cell protein analysis our results showed that studied isolates belonged to “A. sobria complex”, mainly into A. veronii bv. sobria species. Reliable identification of clinical isolates is important for determination of sources of infection.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

Citovat

ČERNOHLÁVKOVÁ, Jitka, Michaela SEDLÁČKOVÁ, Eva KREJČÍ and Ivo SEDLÁČEK. Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material. In ECCMID 2013, Berlin, Germany. 2013.

@proceedings{1104937,
   author = {Černohlávková, Jitka and Sedláčková, Michaela and Krejčí, Eva and Sedláček, Ivo},
   booktitle = {ECCMID 2013, Berlin, Germany},
   keywords = {Aeromonads; classification; biotyping; ribotyping; gene sequencing},
   language = {eng},
   title = {Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material},
   url = {http://www.escmid.org/escmid_library/online_lecture_library/?search=1¤t_page=1&search_term=cernohlavkova},
   year = {2013}
}

TY  - CONF
ID  - 1104937
AU  - Černohlávková, Jitka - Sedláčková, Michaela - Krejčí, Eva - Sedláček, Ivo
PY  - 2013
TI  - Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material
KW  - Aeromonads
KW  - classification
KW  - biotyping
KW  - ribotyping
KW  - gene sequencing
UR  - http://www.escmid.org/escmid_library/online_lecture_library/?search=1¤t_page=1&search_term=cernohlavkova
L2  - http://www.escmid.org/escmid_library/online_lecture_library/?search=1¤t_page=1&search_term=cernohlavkova
N2  - Aeromonas are gram-negative, oxidase positive, fermenting rods widely distributed in the environment. This genus was recognised as an important fish pathogen, however is often associated with gastrointestinal and extraintestinal diseases in human. Despite its clinical significance Aeromonas spp. are not accurately identified during routine clinical examination, because of their phenotypical similarity. We focused on detailed characterisation of 22 clinical isolates of “A. sobria complex” from diarrhoea samples. Biochemical tests were used to classify isolates into “A. sobria complex”. Further, an approach using ribotyping, whole-cell protein analysis and cpn60 gene sequencing were applied for detailed characterisation and identification. Additionally, 11 reference type strains of Aeromonas from Czech Collection of Microorganisms were included in the comparison. Genetic analysis based on ribotyping clearly distinguished the 22 human isolates from reference Aeromonas type strains and clustered them together with A. veronii bv. sobria, A. veronii bv. veronii and A. allosaccharophila reference strains. A. jandaei and A. schubertii were the next closest relation species. Six isolates were clustered into a separate subclaster(similarity 52%), but results of cpn60 gene sequencing of a selected isolate confirmed A. veronii bv. sobria identification. Whole-cell protein analysis showed different distribution of isolates. 16 isolates were grouped together with A. veronii bv. sobria reference strain (similarity level 48 %) and 8 isolates together with A. veronii bv. veronii reference strain. Other isolates were separated from these groups. Despite inconsistent results of ribotying and whole-cell protein analysis our results showed that studied isolates belonged to “A. sobria complex”, mainly into A. veronii bv. sobria species. Reliable identification of clinical isolates is important for determination of sources of infection.
ER  -

ČERNOHLÁVKOVÁ, Jitka, Michaela SEDLÁČKOVÁ, Eva KREJČÍ and Ivo SEDLÁČEK. Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material. In \textit{ECCMID 2013, Berlin, Germany}. 2013.

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