Classification of Aeromonas veronii bv. sobria strains isolated
from human clinical material

ČERNOHLÁVKOVÁ, Jitka, Michaela SEDLÁČKOVÁ, Eva KREJČÍ and Ivo SEDLÁČEK. Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material. In ECCMID 2013, Berlin, Germany. 2013.

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TY  - CONF
ID  - 1104937
AU  - Černohlávková, Jitka - Sedláčková, Michaela - Krejčí, Eva - Sedláček, Ivo
PY  - 2013
TI  - Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material
KW  - Aeromonads
KW  - classification
KW  - biotyping
KW  - ribotyping
KW  - gene sequencing
UR  - http://www.escmid.org/escmid_library/online_lecture_library/?search=1¤t_page=1&search_term=cernohlavkova
L2  - http://www.escmid.org/escmid_library/online_lecture_library/?search=1¤t_page=1&search_term=cernohlavkova
N2  - Aeromonas are gram-negative, oxidase positive, fermenting rods widely distributed in the environment. This genus was recognised as an important fish pathogen, however is often associated with gastrointestinal and extraintestinal diseases in human. Despite its clinical significance Aeromonas spp. are not accurately identified during routine clinical examination, because of their phenotypical similarity. We focused on detailed characterisation of 22 clinical isolates of “A. sobria complex” from diarrhoea samples. Biochemical tests were used to classify isolates into “A. sobria complex”. Further, an approach using ribotyping, whole-cell protein analysis and cpn60 gene sequencing were applied for detailed characterisation and identification. Additionally, 11 reference type strains of Aeromonas from Czech Collection of Microorganisms were included in the comparison. Genetic analysis based on ribotyping clearly distinguished the 22 human isolates from reference Aeromonas type strains and clustered them together with A. veronii bv. sobria, A. veronii bv. veronii and A. allosaccharophila reference strains. A. jandaei and A. schubertii were the next closest relation species. Six isolates were clustered into a separate subclaster(similarity 52%), but results of cpn60 gene sequencing of a selected isolate confirmed A. veronii bv. sobria identification. Whole-cell protein analysis showed different distribution of isolates. 16 isolates were grouped together with A. veronii bv. sobria reference strain (similarity level 48 %) and 8 isolates together with A. veronii bv. veronii reference strain. Other isolates were separated from these groups. Despite inconsistent results of ribotying and whole-cell protein analysis our results showed that studied isolates belonged to “A. sobria complex”, mainly into A. veronii bv. sobria species. Reliable identification of clinical isolates is important for determination of sources of infection.
ER  -

Basic information
Original name	Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material
Name in Czech	Klasifikace kmenů Aeromonas veronii bv. sobria izolovaných z humánního klinického materiálu
Authors	ČERNOHLÁVKOVÁ, Jitka (203 Czech Republic, guarantor, belonging to the institution), Michaela SEDLÁČKOVÁ (203 Czech Republic), Eva KREJČÍ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic).
Edition	ECCMID 2013, Berlin, Germany, 2013.

Other information
Original language	English
Type of outcome	Conference abstract
Field of Study	10600 1.6 Biological sciences
Country of publisher	Czech Republic
Confidentiality degree	is not subject to a state or trade secret
WWW	URL
RIV identification code	RIV/00216224:14310/13:00068202
Organization unit	Faculty of Science
Keywords (in Czech)	aeromonády; klasifikace; biotypizace; ribotypizace; sekvencování
Keywords in English	Aeromonads; classification; biotyping; ribotyping; gene sequencing
Tags	ZR
Tags	International impact, Reviewed
Changed by	Changed by: Ing. Zdeňka Rašková, učo 140529. Changed: 28/4/2014 11:04.

Abstract

Aeromonas are gram-negative, oxidase positive, fermenting rods widely distributed in the environment. This genus was recognised as an important fish pathogen, however is often associated with gastrointestinal and extraintestinal diseases in human. Despite its clinical significance Aeromonas spp. are not accurately identified during routine clinical examination, because of their phenotypical similarity. We focused on detailed characterisation of 22 clinical isolates of “A. sobria complex” from diarrhoea samples. Biochemical tests were used to classify isolates into “A. sobria complex”. Further, an approach using ribotyping, whole-cell protein analysis and cpn60 gene sequencing were applied for detailed characterisation and identification. Additionally, 11 reference type strains of Aeromonas from Czech Collection of Microorganisms were included in the comparison. Genetic analysis based on ribotyping clearly distinguished the 22 human isolates from reference Aeromonas type strains and clustered them together with A. veronii bv. sobria, A. veronii bv. veronii and A. allosaccharophila reference strains. A. jandaei and A. schubertii were the next closest relation species. Six isolates were clustered into a separate subclaster(similarity 52%), but results of cpn60 gene sequencing of a selected isolate confirmed A. veronii bv. sobria identification. Whole-cell protein analysis showed different distribution of isolates. 16 isolates were grouped together with A. veronii bv. sobria reference strain (similarity level 48 %) and 8 isolates together with A. veronii bv. veronii reference strain. Other isolates were separated from these groups. Despite inconsistent results of ribotying and whole-cell protein analysis our results showed that studied isolates belonged to “A. sobria complex”, mainly into A. veronii bv. sobria species. Reliable identification of clinical isolates is important for determination of sources of infection.

Links
EE2.3.20.0183, research and development project	Name: Centrum experimentální biomedicíny

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Classification of Aeromonas veronii bv. sobria strains isolated from human clinical material

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