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@article{1105098,
author = {Kosina, Marcel and Mašlaňová, Ivana and Vávrová Pascutti, Andrea and Šedo, Ondrej and Lexa, Matej and Švec, Pavel and Sedláček, Ivo},
article_location = {Praha},
article_number = {6},
doi = {http://dx.doi.org/10.1007/s12223-013-0236-8},
keywords = {Pseudomonas jessenii subsp. pseudoputida subsp. nov.; phenotyping; ribotyping; rpoB; rrs},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {Description of Pseudomonas jessenii subsp. pseudoputida subsp. nov., amended description of Pseudomonas jessenii and description of Pseudomonas jessenii subsp. jessenii subsp. nov.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23636777},
volume = {58},
year = {2013}
}
TY - JOUR
ID - 1105098
AU - Kosina, Marcel - Mašlaňová, Ivana - Vávrová Pascutti, Andrea - Šedo, Ondrej - Lexa, Matej - Švec, Pavel - Sedláček, Ivo
PY - 2013
TI - Description of Pseudomonas jessenii subsp. pseudoputida subsp. nov., amended description of Pseudomonas jessenii and description of Pseudomonas jessenii subsp. jessenii subsp. nov.
JF - Folia Microbiologica
VL - 58
IS - 6
SP - 631-639
EP - 631-639
PB - Czechoslovak Society for Microbiology
SN - 00155632
KW - Pseudomonas jessenii subsp. pseudoputida subsp. nov.
KW - phenotyping
KW - ribotyping
KW - rpoB
KW - rrs
UR - http://www.ncbi.nlm.nih.gov/pubmed/23636777
L2 - http://www.ncbi.nlm.nih.gov/pubmed/23636777
N2 - Fluorescent Pseudomonas putida CCM 3656 (ATCC 11250) was analysed according to the methods of polyphasic approach which were based on sequence analyses involving the rpoB and rrs genes, manual ribotyping using endonuclease HindIII, DNA base composition determination and DNA-DNA hybridization. The results obtained by these genotyping methods showed that the strain CCM 3656 is distant from P. putida taxon, which was supported with phenotype characterization represented by whole-cell protein profile analysis, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry profiling and extended biotyping. The DNA-DNA hybridization experiments performed between the strain CCM 3656 and the closest relatives revealed 77 % similarity with Pseudomonas jessenii. However, the outcomes of sequencing, ribotyping and phenotype characterization allow distinguishing the studied strain from P. jessenii. On the basis of the obtained taxonomic data, we suggest reclassifying strain CCM 3656 to a novel subspecies of P. jessenii and propose naming P. jessenii subsp. pseudoputida subsp. nov. with CCM 3656T as type strain. Furthermore, we present an amended description of P. jessenii and proposal of P. jessenii subsp. jessenii subsp. nov.
ER -
KOSINA, Marcel, Ivana MAŠLAŇOVÁ, Andrea VÁVROVÁ PASCUTTI, Ondrej ŠEDO, Matej LEXA, Pavel ŠVEC and Ivo SEDLÁČEK. Description of Pseudomonas jessenii subsp. pseudoputida subsp. nov., amended description of Pseudomonas jessenii and description of Pseudomonas jessenii subsp. jessenii subsp. nov. \textit{Folia Microbiologica}. Praha: Czechoslovak Society for Microbiology, 2013, vol.~58, No~6, p.~631-639. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-013-0236-8.
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