The paper deals with the regeneration of agricultural brownfi elds in the Czech Republic. The fi rst part of paper introduces the issue and the most important results of the previous scientifi c researches. The second part describes the goal of paper and methodology of own research activities. The third part brings own analysis of the Czechinvest Agency dataset of the non-regenerated agricultural brownfi elds collected in period 2005 - 2007 updated for the case study area of the South Moravian Region according the dataset of the Regional Development Agency of the South Moravia (2010). The forth part pays attention to origin and potential location of agricultural brownfi elds in the South Moravian Region according the issue is demonstrated by using of the selected indicators showing decrease of agricultural production in this region. The fifth part brings the more detailed analysis of nonregenerated agricultural brownfi elds for the South Moravian Region. The six parts describes the selected examples of the regenerated agricultural brownfi elds. The fi nal part contains discussion of main results and brings some recommendations which could be useful and inspiring for the diff erent groups of stakeholders (e.g. owners, investors, representatives of public administration etc.) who are involved in process of the regeneration of agricultural brownfi elds.