J 2013

Raman Spectroscopy of Proteins and Nucleoproteins

NĚMEČEK, Daniel, Josef ŠTĚPÁNEK and George J. THOMAS

Basic information

Original name

Raman Spectroscopy of Proteins and Nucleoproteins

Authors

NĚMEČEK, Daniel (203 Czech Republic, guarantor, belonging to the institution), Josef ŠTĚPÁNEK (203 Czech Republic) and George J. THOMAS (840 United States of America)

Edition

Current Protocols in Protein Science, John Wiley & Sons, 2013, 1934-3655

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14740/13:00068311

Organization unit

Central European Institute of Technology

DOI

Keywords in English

viral protein; virus assembly; DNA recognition; Raman spectroscopy

Tags

Tags

International impact, Reviewed
Změněno: 11/4/2014 14:38, Olga Křížová

Abstract

V originále

A protein Raman spectrum comprises discrete bands representing vibrational modes of the peptide backbone and its side chains. The spectral positions, intensities, and polarizations of the Raman bands are sensitive to protein secondary, tertiary, and quaternary structures and to side-chain orientations and local environments. In favorable cases, the Raman spectrum serves as an empirical signature of protein three-dimensional structure, intramolecular dynamics, and intermolecular interactions. Quantitative analysis of Raman spectral series can be further boosted by advanced statistical approaches of factor analysis that allow fitting of specific theoretical models while reducing the amount of analyzed data. Here, the strengths of Raman spectroscopy are illustrated by considering recent applications from the authors’ work that address (1) subunit folding and recognition in assembly of the icosahedral bacteriophages, (2) orientations of subunit main chains and side chains in native filamentous viruses, (3) roles of cysteine hydrogen bonding in the folding, assembly, and function of virus structural proteins, and (4) structural determinants of protein/DNA recognition in gene regulatory complexes. Conventional Raman and polarized Raman techniques are surveyed.