Detailed Information on Publication Record
2013
Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica
KOSINA, Marcel, Miloš BARTÁK, Ivana MAŠLAŇOVÁ, Andrea VÁVROVÁ PASCUTTI, Ondrej ŠEDO et. al.Basic information
Original name
Pseudomonas prosekii sp. nov., a Novel Psychrotrophic Bacterium from Antarctica
Authors
KOSINA, Marcel (203 Czech Republic, guarantor, belonging to the institution), Miloš BARTÁK (203 Czech Republic, belonging to the institution), Ivana MAŠLAŇOVÁ (203 Czech Republic, belonging to the institution), Andrea VÁVROVÁ PASCUTTI (203 Czech Republic, belonging to the institution), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Matej LEXA (703 Slovakia, belonging to the institution) and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution)
Edition
Current Microbiology, Springer US, 2013, 0343-8651
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.359
RIV identification code
RIV/00216224:14310/13:00068676
Organization unit
Faculty of Science
UT WoS
000325813400001
Keywords in English
Antarctic; Pseudomonas; Taxonomy; rpoB; rrs; rpoD
Změněno: 13/3/2018 14:21, doc. Ing. Matej Lexa, Ph.D.
Abstract
V originále
During Czech expeditions at James Ross Island, Antarctica, in the years 2007–2009, the bacterial diversity of the genus Pseudomonas was studied. Twelve fluorescent Pseudomonas strains were isolated from various samples and were subjected to a detailed taxonomic study. A polyphasic approach included genotypic and phenotypic analyses. The genotypic analysis involved sequencing of rrs, rpoB and rpoD genes, DNA–DNA hybridization (DDH) studies as well as manual ribotyping using HindIII endonuclease. The phenotypic characterization included conventional tests as well as biotyping using the Biolog system, protein profiling by SDS-PAGE, and MALDI-TOF MS analysis. Our taxonomic study revealed that all isolates belonged to the same Pseudomonas species with psychrotrophic growth not exceeding 37C. The cultures showed a unique position among the phylogenetically related pseudomonads. DDH experiment between the proposed type strain of the antarctic isolates and the closest neighbour P. arsenicoxydans CCM 8423T showed only 40.9–50.1 % similarity, thus confirming that the characterized strains do not belong to the P. arsenicoxydans species. According to the results obtained we propose the name P. prosekii sp. nov. for this novel Pseudomonas taxon with type strain AN/28/1T (=CCM 7990T and LMG 26867T).
Links
| CZ.1.05/1.1.00/02.0068, interní kód MU |
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| LM2010009, research and development project |
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| MSM0021622416, plan (intention) |
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