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@article{1113356,
author = {Rabatinová, Alžbeta and Šanderová, Hana and Jirát Matějčková, Jitka and Korelusová, Jana and Sojka, Luděk and Barvík, Ivan and Papoušková, Veronika and Sklenář, Vladimír and Žídek, Lukáš and Krásný, Libor},
article_location = {Washington},
article_number = {11},
doi = {http://dx.doi.org/10.1128/JB.00188-13},
keywords = {RNA polymerase; delta subunit; regulation; nucleoside triphosphate; gene expression; cell survival},
language = {eng},
issn = {0021-9193},
journal = {Journal of Bacteriology},
title = {The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell},
url = {http://jb.asm.org/content/195/11/2603.full},
volume = {195},
year = {2013}
}
TY - JOUR
ID - 1113356
AU - Rabatinová, Alžbeta - Šanderová, Hana - Jirát Matějčková, Jitka - Korelusová, Jana - Sojka, Luděk - Barvík, Ivan - Papoušková, Veronika - Sklenář, Vladimír - Žídek, Lukáš - Krásný, Libor
PY - 2013
TI - The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell
JF - Journal of Bacteriology
VL - 195
IS - 11
SP - 2603-2611
EP - 2603-2611
PB - American Society for Microbiology
SN - 00219193
KW - RNA polymerase
KW - delta subunit
KW - regulation
KW - nucleoside triphosphate
KW - gene expression
KW - cell survival
UR - http://jb.asm.org/content/195/11/2603.full
L2 - http://jb.asm.org/content/195/11/2603.full
N2 - RNA polymerase (RNAP) is an extensively studied multisubunit enzyme required for transcription of DNA into RNA, yet the delta subunit of RNAP remains an enigmatic protein whose physiological roles have not been fully elucidated. Here, we identify a novel, so far unrecognized function of delta from Bacillus subtilis. We demonstrate that delta affects the regulation of RNAP by the concentration of the initiating nucleoside triphosphate ([iNTP]), an important mechanism crucial for rapid changes in gene expression in response to environmental changes. Consequently, we demonstrate that delta is essential for cell survival when facing a competing strain in a changing environment. Hence, although delta is not essential per se, it is vital for the cell's ability to rapidly adapt and survive in nature. Finally, we show that two other proteins, GreA and YdeB, previously implicated to affect regulation of RNAP by [iNTP] in other organisms, do not have this function in B. subtilis.
ER -
RABATINOVÁ, Alžbeta, Hana ŠANDEROVÁ, Jitka JIRÁT MATĚJČKOVÁ, Jana KORELUSOVÁ, Luděk SOJKA, Ivan BARVÍK, Veronika PAPOUŠKOVÁ, Vladimír SKLENÁŘ, Lukáš ŽÍDEK a Libor KRÁSNÝ. The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell. \textit{Journal of Bacteriology}. Washington: American Society for Microbiology, roč.~195, č.~11, s.~2603-2611. ISSN~0021-9193. doi:10.1128/JB.00188-13. 2013.
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