Detailed Information on Publication Record
2013
Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies
ZOBANÍKOVÁ, Marie, Michal STROUHAL, Lenka MIKALOVÁ, Darina ČEJKOVÁ, Lenka AMBROŽOVÁ et. al.Basic information
Original name
Whole Genome Sequence of the Treponema Fribourg-Blanc: Unspecified Simian Isolate Is Highly Similar to the Yaws Subspecies
Authors
ZOBANÍKOVÁ, Marie (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Lenka MIKALOVÁ (203 Czech Republic, belonging to the institution), Darina ČEJKOVÁ (203 Czech Republic, belonging to the institution), Lenka AMBROŽOVÁ (203 Czech Republic, belonging to the institution), Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Lucinda L. FULTON (840 United States of America), Lei CHEN (840 United States of America), Erica SODERGREN (840 United States of America), George M. WEINSTOCK (840 United States of America) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)
Edition
PLOS Neglected Tropical Diseases, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2013, 1935-2735
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 4.489
RIV identification code
RIV/00216224:14110/13:00065612
Organization unit
Faculty of Medicine
UT WoS
000318153100026
Keywords in English
PALLIDUM SSP PALLIDUM; PATHOGENIC TREPONEMES; GENE-EXPRESSION; SYPHILIS; STRAINS; CONSERVATION; PERTENUE; LIPOPROTEIN; INFECTION; DISEASE
Tags
International impact, Reviewed
Změněno: 14/8/2013 15:26, Ing. Mgr. Věra Pospíšilíková
Abstract
V originále
Background: Unclassified simian strain Treponema Fribourg-Blanc was isolated in 1966 from baboons (Papio cynocephalus)in West Africa. This strain was morphologically indistinguishable from T. pallidum ssp. pallidum or ssp. pertenue strains, and it was shown to cause human infections. Methodology/Principal Findings: To precisely define genetic differences between Treponema Fribourg-Blanc (unclassified simian isolate, FB) and T. pallidum ssp. pertenue strains (TPE), a high quality sequence of the whole Fribourg-Blanc genome was determined with 454-pyrosequencing and Illumina sequencing platforms. Combined average coverage of both methods was greater than 5006. Restriction target sites (n = 1,773), identified in silico, of selected restriction enzymes within the Fribourg-Blanc genome were verified experimentally and no discrepancies were found. When compared to the other three sequenced TPE genomes (Samoa D, CDC-2, Gauthier), no major genome rearrangements were found. The Fribourg- Blanc genome clustered with other TPE strains (especially with the TPE CDC-2 strain), while T. pallidum ssp. pallidum strains clustered separately as well as the genome of T. paraluiscuniculi strain Cuniculi A. Within coding regions, 6 deletions, 5 insertions and 117 substitutions differentiated Fribourg-Blanc from other TPEgenomes. Conclusions/Significance: The Fribourg-Blanc genome showed similar genetic characteristics as other TPE strains. Therefore, we propose to rename the unclassified simian isolate to Treponema pallidum ssp. pertenue strain Fribourg-Blanc. Since the Fribourg-Blanc strain was shown to cause experimental infection in human hosts, non-human primates could serve as possible reservoirs of TPE strains. This could considerably complicate recent efforts to eradicate yaws. Genetic differences specific for Fribourg-Blanc could then contribute for identification of cases of animal-derived yaws infections.
Links
| GAP302/12/0574, research and development project |
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| NT11159, research and development project |
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