ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lenka MIKALOVÁ, George M. WEINSTOCK and David ŠMAJS. Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains. Journal of Medical Microbiology. Reading: Society for General Microbiology, 2013, vol. 62, No 2, p. 196-207. ISSN 0022-2615. doi:10.1099/jmm.0.050658-0.
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Basic information
Original name Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
Authors ČEJKOVÁ, Darina (203 Czech Republic, belonging to the institution), Marie ZOBANÍKOVÁ (203 Czech Republic, belonging to the institution), Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Lenka MIKALOVÁ (203 Czech Republic, belonging to the institution), George M. WEINSTOCK (840 United States of America) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution).
Edition Journal of Medical Microbiology, Reading, Society for General Microbiology, 2013, 0022-2615.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.266
RIV identification code RIV/00216224:14110/13:00065613
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1099/jmm.0.050658-0
UT WoS 000315554000004
Keywords in English article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 15. 4. 2014 16:24.
Abstract
This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/ subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.
Links
GAP302/12/0574, research and development projectName: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema
Investor: Czech Science Foundation
NT11159, research and development projectName: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů
Investor: Ministry of Health of the CR
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