Structure of rrn operons in pathogenic noncultivable
treponemes: sequence but not genomic position of intergenic
spacers correlates with classification of Treponema pallidum
and Treponema paraluiscuniculi strains

J 2013

Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lenka MIKALOVÁ et. al.

Basic information

Original name

Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains

Authors

ČEJKOVÁ, Darina (203 Czech Republic, belonging to the institution), Marie ZOBANÍKOVÁ (203 Czech Republic, belonging to the institution), Petra POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Lenka MIKALOVÁ (203 Czech Republic, belonging to the institution), George M. WEINSTOCK (840 United States of America) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Medical Microbiology, Reading, Society for General Microbiology, 2013, 0022-2615

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.266

RIV identification code

RIV/00216224:14110/13:00065613

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1099/jmm.0.050658-0

UT WoS

000315554000004

Keywords in English

article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi

Abstract

V originále

This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/ subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.

Links

GAP302/12/0574, research and development project

Name: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Czech Science Foundation

NT11159, research and development project

Name: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministry of Health of the CR

Files attached

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Request the author's version of the file

Citovat

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lenka MIKALOVÁ, George M. WEINSTOCK and David ŠMAJS. Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains. Journal of Medical Microbiology. Reading: Society for General Microbiology, 2013, vol. 62, No 2, p. 196-207. ISSN 0022-2615. Available from: https://dx.doi.org/10.1099/jmm.0.050658-0.

@article{1115115,
   author = {Čejková, Darina and Zobaníková, Marie and Pospíšilová, Petra and Strouhal, Michal and Mikalová, Lenka and Weinstock, George M. and Šmajs, David},
   article_location = {Reading},
   article_number = {2},
   doi = {http://dx.doi.org/10.1099/jmm.0.050658-0},
   keywords = {article; bacterial strain; bacterium isolate; controlled study; gene deletion; genotype; nonhuman; operon; polymerase chain reaction; priority journal; RNA sequence; Treponema; Treponema pallidum; Treponema paraluiscuniculi},
   language = {eng},
   issn = {0022-2615},
   journal = {Journal of Medical Microbiology},
   title = {Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains},
   volume = {62},
   year = {2013}
}

TY  - JOUR
ID  - 1115115
AU  - Čejková, Darina - Zobaníková, Marie - Pospíšilová, Petra - Strouhal, Michal - Mikalová, Lenka - Weinstock, George M. - Šmajs, David
PY  - 2013
TI  - Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains
JF  - Journal of Medical Microbiology
VL  - 62
IS  - 2
SP  - 196-207
EP  - 196-207
PB  - Society for General Microbiology
SN  - 00222615
KW  - article
KW  - bacterial strain
KW  - bacterium isolate
KW  - controlled study
KW  - gene deletion
KW  - genotype
KW  - nonhuman
KW  - operon
KW  - polymerase chain reaction
KW  - priority journal
KW  - RNA sequence
KW  - Treponema
KW  - Treponema pallidum
KW  - Treponema paraluiscuniculi
N2  - This study examined the sequences of the two rRNA (rrn) operons of pathogenic non-cultivable treponemes, comprising 11 strains of T. pallidum ssp. pallidum (TPA), five strains of T. pallidum ssp. pertenue (TPE), two strains of T. pallidum ssp. endemicum (TEN), a simian Fribourg-Blanc strain and a rabbit T. paraluiscuniculi (TPc) strain. PCR was used to determine the type of 16S- 23S ribosomal intergenic spacers in the rrn operons from 30 clinical samples belonging to five different genotypes. When compared with the TPA strains, TPc Cuniculi A strain had a 17 bp deletion, and the TPE, TEN and Fribourg-Blanc isolates had a deletion of 33 bp. Other than these deletions, only 17 heterogeneous sites were found within the entire region (excluding the 16S- 23S intergenic spacer region encoding tRNA-Ile or tRNA-Ala). The pattern of nucleotide changes in the rrn operons corresponded to the classification of treponemal strains, whilst two different rrn spacer patterns (Ile/Ala and Ala/Ile) appeared to be distributed randomly across species/ subspecies classification, time and geographical source of the treponemal strains. It is suggested that the random distribution of tRNA genes is caused by reciprocal translocation between repetitive sequences mediated by a recBCD-like system.
ER  -

ČEJKOVÁ, Darina, Marie ZOBANÍKOVÁ, Petra POSPÍŠILOVÁ, Michal STROUHAL, Lenka MIKALOVÁ, George M. WEINSTOCK and David ŠMAJS. Structure of rrn operons in pathogenic noncultivable treponemes: sequence but not genomic position of intergenic spacers correlates with classification of Treponema pallidum and Treponema paraluiscuniculi strains. \textit{Journal of Medical Microbiology}. Reading: Society for General Microbiology, 2013, vol.~62, No~2, p.~196-207. ISSN~0022-2615. Available from: https://dx.doi.org/10.1099/jmm.0.050658-0.

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