Informační systém MU
VALENCIA, Rocio G, Gernot WALKO, Lubomír JANDA, Jiří NOVÁČEK, Eva MIHAILOVSKA, Siegfried REIPERT, Kerstin ANDRA-MAROBELA and Gerhard WICHE. Intermediate filament-associated cytolinker plectin 1c destabilizes microtubules in keratinocytes. Molecular Biology of the Cell. BETHESDA: AMER SOC CELL BIOLOGY, 2013, vol. 24, No 6, p. 768-784. ISSN 1059-1524. doi:10.1091/mbc.E12-06-0488.
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Basic information
Original name Intermediate filament-associated cytolinker plectin 1c destabilizes microtubules in keratinocytes
Authors VALENCIA, Rocio G (40 Austria), Gernot WALKO (40 Austria), Lubomír JANDA (203 Czech Republic, guarantor, belonging to the institution), Jiří NOVÁČEK (203 Czech Republic, belonging to the institution), Eva MIHAILOVSKA (40 Austria), Siegfried REIPERT (40 Austria), Kerstin ANDRA-MAROBELA (40 Austria) and Gerhard WICHE (40 Austria).
Edition Molecular Biology of the Cell, BETHESDA, AMER SOC CELL BIOLOGY, 2013, 1059-1524.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 4.548
RIV identification code RIV/00216224:14740/13:00068909
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1091/mbc.E12-06-0488
UT WoS 000321120600020
Keywords in English SUBSTRATE ADHESION DYNAMICS; EPIDERMOLYSIS-BULLOSA; MUSCULAR-DYSTROPHY; TYROSINE KINASES; VIMENTIN NETWORK; CULTURED-CELLS; PROTEINS MAP-1; GLUCOSE-UPTAKE; ALPHA-TUBULIN; TAU
Tags ok, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 26. 7. 2013 08:09.
Abstract
The transition of microtubules (MTs) from an assembled to a disassembled state plays an essential role in several cellular functions. While MT dynamics are often linked to those of actin filaments, little is known about whether intermediate filaments (IFs) have an influence on MT dynamics. We show here that plectin 1c (P1c), one of the multiple isoforms of the IF-associated cytolinker protein plectin, acts as an MT destabilizer. We found that MTs in P1c-deficient (P1c(-/-)) keratinocytes are more resistant toward nocodazole-induced disassembly and display increased acetylation. In addition, live imaging of MTs in P1c(-/-), as well as in plectin-null, cells revealed decreased MT dynamics. Increased MT stability due to P1c deficiency led to changes in cell shape, increased velocity but loss of directionality of migration, smaller-sized focal adhesions, higher glucose uptake, and mitotic spindle aberrations combined with reduced growth rates of cells. On the basis of ex vivo and in vitro experimental approaches, we suggest a mechanism for MT destabilization in which isoform-specific binding of P1c to MTs antagonizes the MT-stabilizing and assembly-promoting function of MT-associated proteins through an inhibitory function exerted by plectin's SH3 domain. Our results open new perspectives on cytolinker-coordinated IF-MT interaction and its physiological significance.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
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