Apolipoprotein E gene polymorphisms in chronic periodontitis

BOŘILOVÁ LINHARTOVÁ, Petra, Jan MÁCHAL, Jiřina BÁRTOVÁ, Hana POSKEROVÁ, Jan VOKURKA, Antonín FASSMANN and Lydie IZAKOVIČOVÁ HOLLÁ. Apolipoprotein E gene polymorphisms in chronic periodontitis. In FEBS CONGRESS 2013, Sankt Petersburg, Russia, FEBS Journal, vol. 280, suppl. 1, pp. 457. 2013. ISSN 1742-4658.

Basic information

• Original name: Apolipoprotein E gene polymorphisms in chronic periodontitis
• Authors: BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, belonging to the institution), Jan MÁCHAL (203 Czech Republic, belonging to the institution), Jiřina BÁRTOVÁ (203 Czech Republic, belonging to the institution), Hana POSKEROVÁ (203 Czech Republic, belonging to the institution), Jan VOKURKA (203 Czech Republic, belonging to the institution), Antonín FASSMANN (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: FEBS CONGRESS 2013, Sankt Petersburg, Russia, FEBS Journal, vol. 280, suppl. 1, pp. 457. 2013.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.986
• RIV identification code: RIV/00216224:14110/13:00065618
• Organization unit: Faculty of Medicine
• ISSN: 1742-4658
• UT WoS: 000325919202433
• Keywords in English: ApoE; chronic periodontitis; gene polymorphism; microbial colonization

Abstract

Objective: Periodontal diseases can be related to common systemic conditions, and, as recently described, alterations in blood lipid levels. The aim of this study was to analyze two apolipoprotein E (ApoE) gene polymorphisms in patients with chronic periodontitis (CP) in relation to periodontopathic bacteria and lipid levels. Methods: 469 unrelated subjects were included in this case-control study. Genomic DNA of 294 patients with CP and 175 healthy/non-periodontitis controls were genotyped, using the real-time polymerase chain reaction (RT-PCR) method monitored by SYBR Green, for ApoE (rs429358 and rs7412) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray based on a periodontal pathogen detection kit and lipid levels were measured in a subgroup of subjects. Results: There was no evidence for a significant association between ApoE gene polymorphisms and CP (E2/E2 vs. E3/E3: OR=0.63, 95%CI=0.12-3.15; E2/E3 vs. E3/E3: OR=1.61, 95%CI=0.84-3.11; E2/E4 vs. E3/E3: OR=1.25, 95%CI=0.23-6.95; E3/E4 vs. E3/E3: OR=1.11, 95%CI=0.65-1.88; E4/E4 vs. E3/E3: OR=0.63, 95%CI=0.15-2.55; allele E2 vs. allele E3: OR=1.26, 95%CI=0.74-2.14; allele E4 vs. allele E3: OR=0.97, 95%CI=0.63-1.51). Patients with CP had increased total cholesterol and LDL levels compared to controls (P<0.05), however no significant differences were found for triglyceride and HDL levels. Gene variability in the ApoE gene influenced LDL levels marginally (P=0.07) but did not modify total cholesterol, triglyceride and HDL levels or the occurrence of periodontal pathogens in subgingival pockets. Conclusions: In Czech population studied, ApoE genetic variations rs429358 and rs7412 were not associated with susceptibility to and severity of chronic periodontitis or with the presence of periodontopathic bacteria.

Links

• MUNI/A/0888/2012, interní kód MU: Name: Výzkum diagnostických a terapeutických přístupů v predikci onemocnění v orofaciální oblasti

Investor: Masaryk University, Category A

• NT11405, research and development project: Name: Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu

Investor: Ministry of Health of the CR