Paramagnetic Particles Isolation of Influenza Oligonucleotide
Labelled with CdS QDs

J 2013

Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs

KREJČOVÁ, Ludmila, David HYNEK, Pavel KOPEL, Vojtech ADAM, Jaromír HUBÁLEK et. al.

Basic information

Original name

Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs

Authors

KREJČOVÁ, Ludmila, David HYNEK, Pavel KOPEL, Vojtech ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ and René KIZEK

Edition

Chromatographia, 2013, 0009-5893

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.370

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s10337-012-2327-0

UT WoS

000316819600008

Keywords in English

Biosensors; Voltammetry; Automated separation; Nanoparticles; Quantum dots; Hybridization; Virus

Abstract

V originále

In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine-adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 A degrees C, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 mu g mL(-1). The detection limit (3 signal/noise) was estimated as 15 ng mL(-1) of ODN-SH-Cd.

Citovat

KREJČOVÁ, Ludmila, David HYNEK, Pavel KOPEL, Vojtech ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ and René KIZEK. Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs. Chromatographia. 2013, vol. 76, 7-8, p. 355-362. ISSN 0009-5893. Available from: https://dx.doi.org/10.1007/s10337-012-2327-0.

@article{1119846,
   author = {Krejčová, Ludmila and Hynek, David and Kopel, Pavel and Adam, Vojtech and Hubálek, Jaromír and Trnková, Libuše and Kizek, René},
   article_number = {7-8},
   doi = {http://dx.doi.org/10.1007/s10337-012-2327-0},
   keywords = {Biosensors; Voltammetry; Automated separation; Nanoparticles; Quantum dots; Hybridization; Virus},
   language = {eng},
   issn = {0009-5893},
   journal = {Chromatographia},
   title = {Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs},
   volume = {76},
   year = {2013}
}

TY  - JOUR
ID  - 1119846
AU  - Krejčová, Ludmila - Hynek, David - Kopel, Pavel - Adam, Vojtech - Hubálek, Jaromír - Trnková, Libuše - Kizek, René
PY  - 2013
TI  - Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs
JF  - Chromatographia
VL  - 76
IS  - 7-8
SP  - 355-362
EP  - 355-362
SN  - 00095893
KW  - Biosensors
KW  - Voltammetry
KW  - Automated separation
KW  - Nanoparticles
KW  - Quantum dots
KW  - Hybridization
KW  - Virus
N2  - In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine-adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 A degrees C, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 mu g mL(-1). The detection limit (3 signal/noise) was estimated as 15 ng mL(-1) of ODN-SH-Cd.
ER  -

KREJČOVÁ, Ludmila, David HYNEK, Pavel KOPEL, Vojtech ADAM, Jaromír HUBÁLEK, Libuše TRNKOVÁ and René KIZEK. Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs. \textit{Chromatographia}. 2013, vol.~76, 7-8, p.~355-362. ISSN~0009-5893. Available from: https://dx.doi.org/10.1007/s10337-012-2327-0.

Detailed Information on Publication Record