| ZÍTKA, Ondřej, Marketa KOMINKOVA, Sylvie SKALICKOVA, Helena SKUTKOVA, Ivo PROVAZNIK, Tomas ECKSCHLAGER, Marie STIBOROVA, Vojtech ADAM, Libuše TRNKOVÁ a René KIZEK. Hydrodynamic Voltammograms Profiling of Metallothionein Fragment. International Journal of Electrochemical Science. 2012, roč. 7, č. 11, s. 10544-10561. ISSN 1452-3981. |
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@article{1119878,
author = {Zítka, Ondřej and Kominkova, Marketa and Skalickova, Sylvie and Skutkova, Helena and Provaznik, Ivo and Eckschlager, Tomas and Stiborova, Marie and Adam, Vojtech and Trnková, Libuše and Kizek, René},
article_number = {11},
keywords = {Metallothionein; Peptides; Flow Injection Analysis; High Throughput Analysis; Bioinformatics; Bioanalysis, Electrochemistry, Carbon Electrode; Sulfhydryl Group},
language = {eng},
issn = {1452-3981},
journal = {International Journal of Electrochemical Science},
title = {Hydrodynamic Voltammograms Profiling of Metallothionein Fragment},
volume = {7},
year = {2012}
}
TY - JOUR
ID - 1119878
AU - Zítka, Ondřej - Kominkova, Marketa - Skalickova, Sylvie - Skutkova, Helena - Provaznik, Ivo - Eckschlager, Tomas - Stiborova, Marie - Adam, Vojtech - Trnková, Libuše - Kizek, René
PY - 2012
TI - Hydrodynamic Voltammograms Profiling of Metallothionein Fragment
JF - International Journal of Electrochemical Science
VL - 7
IS - 11
SP - 10544-10561
EP - 10544-10561
SN - 14523981
KW - Metallothionein
KW - Peptides
KW - Flow Injection Analysis
KW - High Throughput Analysis
KW - Bioinformatics
KW - Bioanalysis, Electrochemistry, Carbon Electrode
KW - Sulfhydryl Group
N2 - Metallothionein is intracellular metal binding protein. It can bind essential zinc ions and also toxic metals like cadmium. In cancer research the problem of resistance of cancer cells against anticancer drugs was described. In this study we optimized an electrochemical method for exploring the differences in electrochemical signals in dependence on amino acid sequence in metallothionein. For this purpose we selected the number of fragments based on bioinformatic processing, synthesized them and made high throughput electrochemical analysis. Designed electrochemical analysis was based on automated flow injection analysis with amperometric detector using Coulochem III. Detection was carried out by glassy carbon electrode. We measured hydrodynamic voltammograms (HDVs) for 23 selected metallothionein fragments within the potential range from 100 to 1200 mV. We estimated the maxima of oxidation by processing of cumulative HDV. The oxidation maxima were dependent on amino acid composition, whereas fragments from human isoforms and some saltwater fishes' species had the highest maxima (900 mV). On the opposite side fragments of other mammals like horse, mouse and bull had the lowest potential maxima (700 mV). This should be related with the binding capacity for metal ions.
ER -
ZÍTKA, Ondřej, Marketa KOMINKOVA, Sylvie SKALICKOVA, Helena SKUTKOVA, Ivo PROVAZNIK, Tomas ECKSCHLAGER, Marie STIBOROVA, Vojtech ADAM, Libuše TRNKOVÁ a René KIZEK. Hydrodynamic Voltammograms Profiling of Metallothionein Fragment. \textit{International Journal of Electrochemical Science}. 2012, roč.~7, č.~11, s.~10544-10561. ISSN~1452-3981.
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