Suppression of Peptide Sample Losses in Autosampler Vials

J 2013

Suppression of Peptide Sample Losses in Autosampler Vials

STEJSKAL, Karel, David POTĚŠIL and Zbyněk ZDRÁHAL

Basic information

Original name

Suppression of Peptide Sample Losses in Autosampler Vials

Authors

STEJSKAL, Karel (203 Czech Republic, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Proteome Research, Washington, USA, ACS, 2013, 1535-3893

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.001

RIV identification code

RIV/00216224:14740/13:00066299

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/pr400183v

UT WoS

000320298600063

Keywords in English

MASS-SPECTROMETRY ANALYSIS; LIQUID-CHROMATOGRAPHY; RECOVERY; PROTEIN; QUANTIFICATION; OPTIMIZATION; PURIFICATION; SURFACTANTS; ADSORPTION; PROTEOMICS

Abstract

V originále

Protein or peptide sample losses could accompany all steps of the proteomic analysis workflow. We focused on suppression of sample adsorptive losses during sample storage in autosampler vials. We examined suppression capabilities of six different sample injection solutions and seven types of autosampler vial surfaces using a model sample (tryptic digest of six proteins, 1 fmol per protein). While the vial material did not play an essential role, the choice of appropriate composition of sample injection solution reduced adsorptive losses substantially. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (6 M and 1 M) as injection solutions (both acidified with formic acid (FA) (0.1%)) provided the best results in terms of number of significantly identified peptides (p < 0.05). These conclusions were confirmed by analyses of a real sample with intermediate complexity (in-gel digest from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)). Addition of PEG into the real sample solution proved to prevent higher losses, concerning mainly hydrophobic peptides, during up to 48 h storage in the autosampler in comparison with a formic acid solution and even with a solution of highly concentrated urea and thiourea. Using PEG for several months was not accompanied by any adverse effect to the liquid chromatography system.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

GBP206/12/G151, research and development project

Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice

Citovat

STEJSKAL, Karel, David POTĚŠIL and Zbyněk ZDRÁHAL. Suppression of Peptide Sample Losses in Autosampler Vials. Journal of Proteome Research. Washington, USA: ACS, 2013, vol. 12, No 6, p. 3057-3062. ISSN 1535-3893. Available from: https://dx.doi.org/10.1021/pr400183v.

@article{1120957,
   author = {Stejskal, Karel and Potěšil, David and Zdráhal, Zbyněk},
   article_location = {Washington, USA},
   article_number = {6},
   doi = {http://dx.doi.org/10.1021/pr400183v},
   keywords = {MASS-SPECTROMETRY ANALYSIS; LIQUID-CHROMATOGRAPHY; RECOVERY; PROTEIN; QUANTIFICATION; OPTIMIZATION; PURIFICATION; SURFACTANTS; ADSORPTION; PROTEOMICS},
   language = {eng},
   issn = {1535-3893},
   journal = {Journal of Proteome Research},
   title = {Suppression of Peptide Sample Losses in Autosampler Vials},
   url = {http://pubs.acs.org/doi/pdf/10.1021/pr400183v},
   volume = {12},
   year = {2013}
}

TY  - JOUR
ID  - 1120957
AU  - Stejskal, Karel - Potěšil, David - Zdráhal, Zbyněk
PY  - 2013
TI  - Suppression of Peptide Sample Losses in Autosampler Vials
JF  - Journal of Proteome Research
VL  - 12
IS  - 6
SP  - 3057-3062
EP  - 3057-3062
PB  - ACS
SN  - 15353893
KW  - MASS-SPECTROMETRY ANALYSIS
KW  - LIQUID-CHROMATOGRAPHY
KW  - RECOVERY
KW  - PROTEIN
KW  - QUANTIFICATION
KW  - OPTIMIZATION
KW  - PURIFICATION
KW  - SURFACTANTS
KW  - ADSORPTION
KW  - PROTEOMICS
UR  - http://pubs.acs.org/doi/pdf/10.1021/pr400183v
L2  - http://pubs.acs.org/doi/pdf/10.1021/pr400183v
N2  - Protein or peptide sample losses could accompany all steps of the proteomic analysis workflow. We focused on suppression of sample adsorptive losses during sample storage in autosampler vials. We examined suppression capabilities of six different sample injection solutions and seven types of autosampler vial surfaces using a model sample (tryptic digest of six proteins, 1 fmol per protein). While the vial material did not play an essential role, the choice of appropriate composition of sample injection solution reduced adsorptive losses substantially. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (6 M and 1 M) as injection solutions (both acidified with formic acid (FA) (0.1%)) provided the best results in terms of number of significantly identified peptides (p < 0.05). These conclusions were confirmed by analyses of a real sample with intermediate complexity (in-gel digest from sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)). Addition of PEG into the real sample solution proved to prevent higher losses, concerning mainly hydrophobic peptides, during up to 48 h storage in the autosampler in comparison with a formic acid solution and even with a solution of highly concentrated urea and thiourea. Using PEG for several months was not accompanied by any adverse effect to the liquid chromatography system.
ER  -

STEJSKAL, Karel, David POTĚŠIL and Zbyněk ZDRÁHAL. Suppression of Peptide Sample Losses in Autosampler Vials. \textit{Journal of Proteome Research}. Washington, USA: ACS, 2013, vol.~12, No~6, p.~3057-3062. ISSN~1535-3893. Available from: https://dx.doi.org/10.1021/pr400183v.

Detailed Information on Publication Record