Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels

KONEČNÁ, Hana, Danuše FRIDRICHOVÁ, Gabriela LOCHMANOVÁ, Jan BÁRTA and Zbyněk ZDRÁHAL. Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels. Electrophoresis. Hoboken: WILEY-BLACKWELL, 2013, vol. 34, No 13, p. 1969-1971. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.201300084.

Basic information

• Original name: Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels
• Authors: KONEČNÁ, Hana (203 Czech Republic, belonging to the institution), Danuše FRIDRICHOVÁ (203 Czech Republic, belonging to the institution), Gabriela LOCHMANOVÁ (203 Czech Republic, belonging to the institution), Jan BÁRTA (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Electrophoresis, Hoboken, WILEY-BLACKWELL, 2013, 0173-0835.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.161
• RIV identification code: RIV/00216224:14740/13:00065684
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1002/elps.201300084
• UT WoS: 000322112200017
• Keywords in English: 2DE; Mixed detergent micelles; Tris-Tricine gel
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Application of Tris-N-[Tris(hydroxymethyl)methyl]glycine gels for 2DE is hampered by formation of mixed CHAPS-SDS micelles resulting in typical swirling pattern in the low mass range, which makes reliable quantitative and qualitative gel evaluation impossible. Modification of 2DE strip equilibration procedure prevented the direct interaction between both detergents during equilibration process, thus substantially improving gel separation.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• GBP206/12/G151, research and development project: Name: Centrum nových přístupů k bioanalýze a molekulární diagnostice
• QI91A069, research and development project: Name: Hodnocení potenciálu proteinů planých genotypů brambor pro využití ve šlechtění bramboru (Solanum tuberosum L.) a v biotechnologiích.

Investor: Ministry of Agriculture of the CR, Evaluation of wild potato tuber proteins potential for utilization in potato (Solanum tuberosum L.) breeding and biotechnological applications, Sustainable development of agrarian sector