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@article{1121021,
author = {Kšicová, Kateřina and Dušková, Marta and Karpíšková, Renata},
article_number = {2},
keywords = {: restriction analysis of the 16S rDNA gene; aluI; MspI; in silico fragmentation; species identification},
language = {eng},
issn = {1212-1800},
journal = {Czech Journal of Food Science},
title = {Differentiation of Lactobacillus Species by ARDRA},
volume = {31},
year = {2013}
}
TY - JOUR
ID - 1121021
AU - Kšicová, Kateřina - Dušková, Marta - Karpíšková, Renata
PY - 2013
TI - Differentiation of Lactobacillus Species by ARDRA
JF - Czech Journal of Food Science
VL - 31
IS - 2
SP - 180-188
EP - 180-188
SN - 12121800
KW - : restriction analysis of the 16S rDNA gene
KW - aluI
KW - MspI
KW - in silico fragmentation
KW - species identification
N2 - The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (aluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.
ER -
KŠICOVÁ, Kateřina, Marta DUŠKOVÁ a Renata KARPÍŠKOVÁ. Differentiation of Lactobacillus Species by ARDRA. \textit{Czech Journal of Food Science}. 2013, roč.~31, č.~2, s.~180-188. ISSN~1212-1800.
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