Differentiation of Lactobacillus Species by ARDRA

J 2013

Differentiation of Lactobacillus Species by ARDRA

KŠICOVÁ, Kateřina, Marta DUŠKOVÁ and Renata KARPÍŠKOVÁ

Basic information

Original name

Differentiation of Lactobacillus Species by ARDRA

Authors

KŠICOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Marta DUŠKOVÁ (203 Czech Republic, guarantor) and Renata KARPÍŠKOVÁ (203 Czech Republic)

Edition

Czech Journal of Food Science, 2013, 1212-1800

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.741

RIV identification code

RIV/00216224:14310/13:00069157

Organization unit

Faculty of Science

UT WoS

000318259400011

Keywords in English

: restriction analysis of the 16S rDNA gene; aluI; MspI; in silico fragmentation; species identification

Abstract

V originále

The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (aluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

KŠICOVÁ, Kateřina, Marta DUŠKOVÁ and Renata KARPÍŠKOVÁ. Differentiation of Lactobacillus Species by ARDRA. Czech Journal of Food Science. 2013, vol. 31, No 2, p. 180-188. ISSN 1212-1800.

@article{1121021,
   author = {Kšicová, Kateřina and Dušková, Marta and Karpíšková, Renata},
   article_number = {2},
   keywords = {: restriction analysis of the 16S rDNA gene; aluI; MspI; in silico fragmentation; species identification},
   language = {eng},
   issn = {1212-1800},
   journal = {Czech Journal of Food Science},
   title = {Differentiation of Lactobacillus Species by ARDRA},
   volume = {31},
   year = {2013}
}

TY  - JOUR
ID  - 1121021
AU  - Kšicová, Kateřina - Dušková, Marta - Karpíšková, Renata
PY  - 2013
TI  - Differentiation of Lactobacillus Species by ARDRA
JF  - Czech Journal of Food Science
VL  - 31
IS  - 2
SP  - 180-188
EP  - 180-188
SN  - 12121800
KW  - : restriction analysis of the 16S rDNA gene
KW  - aluI
KW  - MspI
KW  - in silico fragmentation
KW  - species identification
N2  - The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (aluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.
ER  -

KŠICOVÁ, Kateřina, Marta DUŠKOVÁ and Renata KARPÍŠKOVÁ. Differentiation of Lactobacillus Species by ARDRA. \textit{Czech Journal of Food Science}. 2013, vol.~31, No~2, p.~180-188. ISSN~1212-1800.

Detailed Information on Publication Record