J
2013
Differentiation of Lactobacillus Species by ARDRA
KŠICOVÁ, Kateřina, Marta DUŠKOVÁ and Renata KARPÍŠKOVÁ
Basic information
Original name
Differentiation of Lactobacillus Species by ARDRA
Authors
KŠICOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Marta DUŠKOVÁ (203 Czech Republic, guarantor) and Renata KARPÍŠKOVÁ (203 Czech Republic)
Edition
Czech Journal of Food Science, 2013, 1212-1800
Other information
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.741
RIV identification code
RIV/00216224:14310/13:00069157
Organization unit
Faculty of Science
Keywords in English
: restriction analysis of the 16S rDNA gene; aluI; MspI; in silico fragmentation; species identification
V originále
The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (aluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.
Links
MSM0021622415, plan (intention) | Name: Molekulární podstata buněčných a tkáňových regulací | Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations |
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