KŠICOVÁ, Kateřina, Marta DUŠKOVÁ and Renata KARPÍŠKOVÁ. Differentiation of Lactobacillus Species by ARDRA. Czech Journal of Food Science. 2013, vol. 31, No 2, p. 180-188. ISSN 1212-1800.
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Basic information
Original name Differentiation of Lactobacillus Species by ARDRA
Authors KŠICOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Marta DUŠKOVÁ (203 Czech Republic, guarantor) and Renata KARPÍŠKOVÁ (203 Czech Republic).
Edition Czech Journal of Food Science, 2013, 1212-1800.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.741
RIV identification code RIV/00216224:14310/13:00069157
Organization unit Faculty of Science
UT WoS 000318259400011
Keywords in English : restriction analysis of the 16S rDNA gene; aluI; MspI; in silico fragmentation; species identification
Tags AKR, rivok, ZR
Changed by Changed by: Ing. Zdeňka Rašková, učo 140529. Changed: 28/4/2014 14:57.
Abstract
The Lactobacillus species by 16S Amplified Ribosomal DNA Restriction Analysis (16S-ARDRA) was identified. Lactobacilli are bacteria often found in foodstuffs of both animal and vegetable origins. On one hand, they play an important role in the food spoilage and, on the other hand, they are used as starter cultures in food fermentation processes. The species-specific identification by traditional biochemical methods is time consuming and not always fully effective. Therefore, more efficient techniques are searched for. We focused on rapid identification of Lactobacillus isolates from different habitats. Forty-nine collection strains and isolates belonging to the genus Lactobacillus were discriminated. ARDRA was carried out with two restriction endonucleases. For the comparison of similarity, the Jaccard coefficient and clustering by the unweighted pair group method with arithmetic averages (UPGMA) were used. The percentages of similarity between profiles varied from 22% to 100% (aluI) and from 27% to 100% (MspI). This method proved applicable to the differentiation of 10 species.
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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