Genome-wide transcriptional analysis of Drosophila larvae
infected by entomopathogenic nematodes shows involvement of
complement, recognition and extracellular matrix proteins

J 2014

Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins

AREFIN, Badrul, Lucie KUČEROVÁ, Pavel DOBEŠ, Robert MARKUS, Hynek STRNAD et. al.

Basic information

Original name

Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins

Authors

AREFIN, Badrul (50 Bangladesh), Lucie KUČEROVÁ (203 Czech Republic), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Robert MARKUS (348 Hungary), Hynek STRNAD (203 Czech Republic), Zhi WANG (156 China), Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution), Michal ŽUROVEC (203 Czech Republic) and Ulrich THEOPOLD (752 Sweden)

Edition

Journal of Innate Immunity, Basel, Karger Publishers, 2014, 1662-811X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30102 Immunology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.352

RIV identification code

RIV/00216224:14310/14:00074739

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1159/000353734

UT WoS

000331774900009

Keywords in English

Basement membrane; Coagulation; Hemocyte; Insect immunity; Nematodes; Recognition molecule; Thioester-containing proteins

Abstract

V originále

Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

AREFIN, Badrul, Lucie KUČEROVÁ, Pavel DOBEŠ, Robert MARKUS, Hynek STRNAD, Zhi WANG, Pavel HYRŠL, Michal ŽUROVEC and Ulrich THEOPOLD. Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins. Journal of Innate Immunity. Basel: Karger Publishers, 2014, vol. 6, No 2, p. 192-204. ISSN 1662-811X. Available from: https://dx.doi.org/10.1159/000353734.

@article{1121079,
   author = {Arefin, Badrul and Kučerová, Lucie and Dobeš, Pavel and Markus, Robert and Strnad, Hynek and Wang, Zhi and Hyršl, Pavel and Žurovec, Michal and Theopold, Ulrich},
   article_location = {Basel},
   article_number = {2},
   doi = {http://dx.doi.org/10.1159/000353734},
   keywords = {Basement membrane; Coagulation; Hemocyte; Insect immunity; Nematodes; Recognition molecule; Thioester-containing proteins},
   language = {eng},
   issn = {1662-811X},
   journal = {Journal of Innate Immunity},
   title = {Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins},
   url = {http://www.karger.com/Article/FullText/353734},
   volume = {6},
   year = {2014}
}

TY  - JOUR
ID  - 1121079
AU  - Arefin, Badrul - Kučerová, Lucie - Dobeš, Pavel - Markus, Robert - Strnad, Hynek - Wang, Zhi - Hyršl, Pavel - Žurovec, Michal - Theopold, Ulrich
PY  - 2014
TI  - Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins
JF  - Journal of Innate Immunity
VL  - 6
IS  - 2
SP  - 192-204
EP  - 192-204
PB  - Karger Publishers
SN  - 1662811X
KW  - Basement membrane
KW  - Coagulation
KW  - Hemocyte
KW  - Insect immunity
KW  - Nematodes
KW  - Recognition molecule
KW  - Thioester-containing proteins
UR  - http://www.karger.com/Article/FullText/353734
L2  - http://www.karger.com/Article/FullText/353734
N2  - Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses.
ER  -

AREFIN, Badrul, Lucie KUČEROVÁ, Pavel DOBEŠ, Robert MARKUS, Hynek STRNAD, Zhi WANG, Pavel HYRŠL, Michal ŽUROVEC and Ulrich THEOPOLD. Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins. \textit{Journal of Innate Immunity}. Basel: Karger Publishers, 2014, vol.~6, No~2, p.~192-204. ISSN~1662-811X. Available from: https://dx.doi.org/10.1159/000353734.

Detailed Information on Publication Record