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@article{1121893,
author = {Bocková, Eva and Rudolf, Ivo and Kočišová, Alica and Betášová, Lenka and Venclíková, Kristýna and Mendel, Jan and Hubálek, Zdeněk},
article_number = {10},
doi = {http://dx.doi.org/10.1007/s00436-013-3526-9},
keywords = {Aedes vexans; microfilariae; Dirofilaria repens; Dirofilaria immitis; canine dirofilariosis; Slovakia},
language = {eng},
issn = {0932-0113},
journal = {Parasitology Research},
title = {Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia},
volume = {112},
year = {2013}
}
TY - JOUR
ID - 1121893
AU - Bocková, Eva - Rudolf, Ivo - Kočišová, Alica - Betášová, Lenka - Venclíková, Kristýna - Mendel, Jan - Hubálek, Zdeněk
PY - 2013
TI - Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia
JF - Parasitology Research
VL - 112
IS - 10
SP - 3465-3470
EP - 3465-3470
SN - 09320113
KW - Aedes vexans
KW - microfilariae
KW - Dirofilaria repens
KW - Dirofilaria immitis
KW - canine dirofilariosis
KW - Slovakia
N2 - t In this study, we screened field-caught mosquitoes for presence of Dirofilaria spp. by using a polymerase chain reaction (PCR) assay. Potential occurrence of Dirofilaria repens and Dirofilaria immitis microfilariae was examined in 3,600 mosquitoes of eight species (Aedes vexans, Aedes cinereus, Aedes rossicus, Culex pipiens, Culiseta annulata, Ochlerotatus sticticus, Ochlerotatus cantans and Ochlerotatus caspius) collected from five locations in two districts (Kosice and Trebisov) of Eastern Slovakia, endemic region of canine dirofilariasis. Collection of mosquitoes was performed between May and August 2012 in premises known to be inhabited by Dirofilaria-infected dogs. PCR assays were performed on 72 pools, each pool containing 50 mosquitoes of the same species, collected on the same location. Each pool was examined separately for the presence of D. immitis and D. repens, respectively. A positive finding ofD. repenswas recorded in one pool of A. vexans mosquitoes collected in Košické Olšany village. Minimum infection rate in A. vexans was 1:1,750, i.e. 0.57 per 1,000 mosquitoes. The identity of D. repens was confirmed by direct sequencing of PCR product which has shown 100 % homology with sequence attributed to D. repens (GenBank accession number AJ271614). This study represents the first molecular evidence of D. repensmicrofilariae in mosquitoes in Slovakia and highlights a need for better surveillance of zoonotic dirofilariasis in central Europe.
ER -
BOCKOVÁ, Eva, Ivo RUDOLF, Alica KOČIŠOVÁ, Lenka BETÁŠOVÁ, Kristýna VENCLÍKOVÁ, Jan MENDEL and Zdeněk HUBÁLEK. Dirofilaria repens microfilariae in Aedes vexans mosquitoes in Slovakia. \textit{Parasitology Research}. 2013, vol.~112, No~10, p.~3465-3470. ISSN~0932-0113. Available from: https://dx.doi.org/10.1007/s00436-013-3526-9.
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